The recessive buff (Vps33abf) mutation arose spontaneously in 1962 on the C57BL/6J background at The Jackson Laboratory. It was maintained primarily by sibling breeding heterozygote x homozygote and in later years the strain was also intermittently backcrossed homozygote to C57BL/6J. In 1975 this strain was at generation N7F24 then N8, in 1977 it reached generation N9F3, in 1979 N9F7 then N10, and in 1981 heterozygous embryos were frozen from C57BL/6J females bred to homozygous males at N10F4 or N10F5.
|Gene Symbol and Name||Vps33a, vacuolar protein sorting 33A (yeast)|
|Gene Synonym(s)||3830421M04Rik; 3830421M04Rik; AI503300; AW048546; AW554476; RIKEN cDNA 3830421M04 gene; bf; bf; buff; expressed sequence AI503300; expressed sequence AW048546; expressed sequence AW554476; r-Vps33a|
|Strain of Origin||C57BL/6J|