This ENU induced mutation causes lethality in homozygous embryos by day E13.5, and heterozygous mice exhibit eye defects and curly vibrissae.
Bruce Beutler, University of Texas Southwestern Medical
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Chemically induced (ENU) (Not Applicable) | Egfr | epidermal growth factor receptor |
Mice homozygous for this mutation have an embryonic lethal phenotype, failing to develop past embryonic days 13.5. Heterozygous mice are viable, fertile, do not display any behavioral abnormalities and are born with open eyelids and curly vibrissae. Adult heterozygotes often have small eyes and corneal opacity with excessive secretions at the eyelid edges. MEFs (mouse embryonic fibroblasts) exhibit reduced migratory ability, approximately 53% of wildtype controls. Histological analysis of homozygous E12.5 embryos reveals placental defects. This mutant mouse strain may be useful in studies of development and as a visible dominant marker for the Egfr allelic series.
This strain was developed using ENU mutagenesis of male C57BL/6J mice. This mutation was identified in F1 offspring of the ENU-treated male founder. The ENU treatment induced an adenine-to-guanine transition, leading to an amino acid change from aspartic acid to glycine at position 833 of the protein (D833G). The D833G mutation is the same mutation identified in EgfrWa5. Mutant animals were backcrossed to C57BL/6J for at least 10 generations.
Allele Name | velvet |
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Allele Type | Chemically induced (ENU) (Not Applicable) |
Allele Synonym(s) | EgfrM1Btlr |
Gene Symbol and Name | Egfr, epidermal growth factor receptor |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 11 |
General Note | This allele contains the same point mutation as in EgfrWa5 in a different ENU screen and strain. Both alleles are considered antimorphs. |
Molecular Note | An A to G transition in the coding region causes the replacement of aspartic acid by glycine at position 833 (D833G). The mutation causes an alteration in the cytoplasmic tyrosine kinase domain structure and falls within a DFG motif known to be important for ATP coordination. |
Mutations Made By | Bruce Beutler, University of Texas Southwestern Medical |
When maintaining a live colony, these mice are bred as heterozygotes. Homozygotes have an embryonic lethal phenotype.
When using the Velvet mouse strain in a publication, please cite the originating article(s) and include JAX stock #006926 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Egfr<Vel> |
Frozen Mouse Embryo | C57BL/6J-Egfr<Vel>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Egfr<Vel>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6J-Egfr<Vel>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6J-Egfr<Vel>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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