These mutant mice may be useful in studying the role of Crkl tyrosine-phosphorylation in Bcr/Abl (Philadelphia chromosome) chronic myelogenous leukemia (CML) and acute lymphoblastic leukemia (ALL), as well as Digeorge Syndrome (DGS) and Velocardiofacial Syndrome.
Nora Heisterkamp, Childrens Hospital Los Angeles
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Crkl | v-crk avian sarcoma virus CT10 oncogene homolog-like |
While heterozygous mice are viable and fertile, mice homozygous for this targeted allele die in utero. Immunoblots from homozygous tissues show no protein expression from the targeted gene. The prenatal lethality exhibited by homozygotes on this C57BL/6J congenic background (and also on a 129Sv genetic background) likely results from heart, liver, and placental defects. Please note that homozygous mutants on a mixed/outbred genetic background (129/Sv X Black Swiss) are viable and fertile. These mutant mice may be useful in studying the role of Crkl tyrosine-phosphorylation in Bcr/Abl (Philadelphia chromosome) chronic myelogenous leukemia (CML), acute lymphoblastic leukemia (ALL), Digeorge Syndrome (DGS) and Velocardiofacial Syndrome.
A targeting vector was designed to replace a portion of exon 1 of the targeted gene with a neomycin resistance cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected into blastocysts, and chimeric offspring were bred to 129X1/SvJ mice. Heterozygous mutants were then backcrossed to C57BL/6J for at least 12 generations prior to arrival at The Jackson Laboratory.
Allele Name | targeted mutation 1, Nora Heisterkamp |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | crkl- |
Gene Symbol and Name | Crkl, v-crk avian sarcoma virus CT10 oncogene homolog-like |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 16 |
Molecular Note | A portion of exon 1 was replaced with a neomycin selection cassette inserted by homologous recombination. |
Mutations Made By | Nora Heisterkamp, Childrens Hospital Los Angeles |
When maintaining a live colony, heterozygous mice may be bred to wildtype siblings or C57BL/6J inbred mice. Homozygous mice on this genetic background die in utero.
When using the B6.129-Crkltm1Hkp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006910 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Crkl<tm1Hkp> |
Frozen Mouse Embryo | B6.129-Crkl<tm1Hkp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Crkl<tm1Hkp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Crkl<tm1Hkp>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Crkl<tm1Hkp>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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