These Msctm1Eno (formerly MyoR-) knock-out mice show no obvious abnormalities, but in combination with other mutants may show craniofacial and other muscle abnormalities.
Rhonda Bassel-Duby, University of Texas Southwestern
Mice homozygous for this MyoR mutant allele are viable and fertile with no obvious abnormalities. These mice may be useful in studying muscle development, specifically craniofacial muscles.
For example, when these mice are bred with capsulin-mutant mice, the resulting double mutant offspring have significant abnormalities in craniofacial (and other) muscle development and MyoD-family transcription factor gene expression.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was designed replace the coding region within the first exon of the targeted gene with a neomyocin-resistance gene in the opposite orientation. The construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were bred to generate the mutant colony on a 129 genetic background. At some point, these mice were bred with C57BL/6 mice. Homozygous mice on a mixed C57BL/6 and 129 genetic background were bred together for many generations prior to arrival at The Jackson Laboratory.
|Allele Name||targeted mutation 1, Eric N Olson|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Msc, musculin|
|Strain of Origin||129|
|Molecular Note||Gene targeting resulted in deletion of the coding region within the first exon and introduced a neomyocin-resistance gene in the opposite orientation to the gene.|
|Mutations Made By|| |
Eric Olson, University of Texas Southwestern Medical
When maintaining a live colony, homozygous mice are bred.
When using the MyoR- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006904 in your Materials and Methods section.