B6.129-Mgat4b^tm1Jxm/J

Stock number: 006903
Research areas: Research Tools

Description

Mgat4bF floxed mice possess loxP sites flanking exons 2-4 of the Mgat4b gene. When bred to a cre-expressing strain, the exon is excised in tissues of the resulting offspring where cre activity is present. The recombined allele generates a truncated GnT-IVb protein lacking a catalytic domain. This strain may be useful for generating conditional mutations in applications related to the regulation of endosomal sorting and autophagy.

Detailed description

Mice that are homozygous for the floxed targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to a cre-expressing strain, the exon is excised in tissues of the resulting offspring where cre activity is present. The recombined allele generates a truncated GnT-IVb protein lacking a catalytic domain.

Development

A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of exons 2, 3, and 4, and a loxP site upstream of the exons. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with Cre expression plasmid to excise the neo fragment and leave the floxed exons. Gancyclovir-resistant ES cells were injected into blastocysts. Chimeras were backcrossed to C57BL/6 at least 9 times by the donating laboratory.

Allele

Symbol: Mgat4b^tm1Jxm
Name: targeted mutation 1, Jamey Marth
MGI accession ID: MGI:3699595
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Mgat4b
Marker name: mannoside acetylglucosaminyltransferase 4, isoenzyme B
Chromosome: 11
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of exons 2, 3, and 4, and a loxP site upstream of the exons. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with Cre expression plasmid to excise the neo fragment and leave the floxed exons.