Mgat4bF floxed mice possess loxP sites flanking exons 2-4 of the Mgat4b gene. When bred to a cre-expressing strain, the exon is excised in tissues of the resulting offspring where cre activity is present. The recombined allele generates a truncated GnT-IVb protein lacking a catalytic domain. This strain may be useful for generating conditional mutations in applications related to the regulation of endosomal sorting and autophagy.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Mgat4b | mannoside acetylglucosaminyltransferase 4, isoenzyme B |
Mice that are homozygous for the floxed targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to a cre-expressing strain, the exon is excised in tissues of the resulting offspring where cre activity is present. The recombined allele generates a truncated GnT-IVb protein lacking a catalytic domain.
A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of exons 2, 3, and 4, and a loxP site upstream of the exons. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with Cre expression plasmid to excise the neo fragment and leave the floxed exons. Gancyclovir-resistant ES cells were injected into blastocysts. Chimeras were backcrossed to C57BL/6 at least 9 times by the donating laboratory.
Allele Name | targeted mutation 1, Jamey Marth |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Mgat4b type II; Mgat4bF |
Gene Symbol and Name | Mgat4b, mannoside acetylglucosaminyltransferase 4, isoenzyme B |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 11 |
Molecular Note | A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of exons 2, 3, and 4, and a loxP site upstream of the exons. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with Cre expression plasmid to excise the neo fragment and leave the floxed exons. |
When maintaining a live colony, homozygotes may be bred successfully.
When using the B6.129-Mgat4btm1Jxm/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006903 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Mgat4b<tm1Jxm> |
Frozen Mouse Embryo | B6.129-Mgat4b<tm1Jxm>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Mgat4b<tm1Jxm>/J | $2595.00 |
Frozen Mouse Embryo | B6.129-Mgat4b<tm1Jxm>/J | $3373.50 |
Frozen Mouse Embryo | B6.129-Mgat4b<tm1Jxm>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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