B6.129-St6gal1^tm2Jxm/J

Stock number: 006901
Research areas: Research Tools

Description

Mice homozygous for this beta galactoside alpha 2,6 sialyltransferase 1 floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.

Detailed description

Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.

Development

A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette in the intron upstream of exon 2 and a loxP site in the intron downstream of exon 2. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with a Cre expression plasmid to excise the neo cassette, leaving the floxed exon. Gancyclovir-resistant ES cells were injected into blastocysts. Chimeric animals were crossed with C57BL/6 by the donating laboratory for a minimum of six generations.

Allele

Symbol: St6gal1^tm2Jxm
Name: targeted mutation 2, Jamey Marth
MGI accession ID: MGI:3699718
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: ST6Gal^F
Marker symbol: St6gal1
Marker name: beta galactoside alpha 2,6 sialyltransferase 1
Marker synonyms: CDw75; Siat1; ST6Gal I; ST6GalI; St6Gal-I; ST6N
Chromosome: 16
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette upstream of exon 2 and a lox P site downstream of the exon. ES cells were transfected with a Cre expression plasmid to excise the neo cassette, leaving the floxed exon.