Mice homozygous for this beta galactoside alpha 2,6 sialyltransferase 1 floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | St6gal1 | beta galactoside alpha 2,6 sialyltransferase 1 |
Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.
A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette in the intron upstream of exon 2 and a loxP site in the intron downstream of exon 2. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with a Cre expression plasmid to excise the neo cassette, leaving the floxed exon. Gancyclovir-resistant ES cells were injected into blastocysts. Chimeric animals were crossed with C57BL/6 by the donating laboratory for a minimum of six generations.
Allele Name | targeted mutation 2, Jamey Marth |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | ST6GalF |
Gene Symbol and Name | St6gal1, beta galactoside alpha 2,6 sialyltransferase 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 16 |
Molecular Note | A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette upstream of exon 2 and a lox P site downstream of the exon. ES cells were transfected with a Cre expression plasmid to excise the neo cassette, leaving the floxed exon. |
Mutations Made By | Jamey Marth, Burnham Inst at Univ Calif Santa Barbara |
Homozygotes are viable and fertile. When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129-St6gal1tm2Jxm/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006901 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for St6gal1<tm2Jxm> |
Frozen Mouse Embryo | B6.129-St6gal1<tm2Jxm>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-St6gal1<tm2Jxm>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-St6gal1<tm2Jxm>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-St6gal1<tm2Jxm>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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