These St3gal3 knock-out mice may be useful for studies related to epilepsy and inflammation.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | St3gal3 | ST3 beta-galactoside alpha-2,3-sialyltransferase 3 |
Mice homozygous for this targeted mutation frequently develop neurological symptoms of spontaneous non-lethal epileptic seizures, dermatitis, and failure-to-thrive.
A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of the exon encoding the transmembrane domain and a loxP site upstream of the exon. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl +-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with a Cre expression plasmid to excise the floxed regions. Gancyclovir-resistant ES cells were injected into blastocysts. Heterozygotes were crossed with ZP3-Cre transgenic mates to produce the recombined and deleted Type 1 allele in the germline. This strain was backcrossed with C57BL/6 mates by the donating laboratory at least nine times.
Allele Name | targeted mutation 1, Jamey Marth |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | ST3Gal-IIIdelta |
Gene Symbol and Name | St3gal3, ST3 beta-galactoside alpha-2,3-sialyltransferase 3 |
Gene Synonym(s) | |
Strain of Origin | Not Specified |
Chromosome | 4 |
Molecular Note | The exon encoding the transmembrane domain was replaced with a single loxP site after the insertion of loxP sites and subsequent expression of cre recombinase in ES cells. |
Mutations Made By | Jamey Marth, Burnham Inst at Univ Calif Santa Barbara |
When maintained as a live colony, heterozygous crosses may be used. Homozygotes develop epilepsy and inflammation of the skin.
When using the ST3Gal-III KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006899 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for St3gal3<tm1Jxm> |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.