B6.129-St3gal3^tm1Jxm/J

Stock number: 006899
Product name: ST3Gal-III KO
Research areas: Immunology, Inflammation and Autoimmunity Research, Neurobiology Research

Description

These St3gal3 knock-out mice may be useful for studies related to epilepsy and inflammation.

Detailed description

Mice homozygous for this targeted mutation frequently develop neurological symptoms of spontaneous non-lethal epileptic seizures, dermatitis, and failure-to-thrive.

Development

A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of the exon encoding the transmembrane domain and a loxP site upstream of the exon. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl ⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with a Cre expression plasmid to excise the floxed regions. Gancyclovir-resistant ES cells were injected into blastocysts. Heterozygotes were crossed with ZP3-Cre transgenic mates to produce the recombined and deleted Type 1 allele in the germline. This strain was backcrossed with C57BL/6 mates by the donating laboratory at least nine times.

Allele

Symbol: St3gal3^tm1Jxm
Name: targeted mutation 1, Jamey Marth
MGI accession ID: MGI:2445404
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: St3gal3
Marker name: ST3 beta-galactoside alpha-2,3-sialyltransferase 3
Chromosome: 4
Strain of origin: Not Specified
Molecular note: The exon encoding the transmembrane domain was replaced with a single loxP site after the insertion of loxP sites and subsequent expression of cre recombinase in ES cells.