These floxed mutant mice possess loxP sites flanking exon 3 of the Galnt1 gene. This strain may be useful for generating conditional mutations in applications related to humoral immunity.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
Mice homozygous for this floxed targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon is deleted by cre expression to produce a null allele.
A targeting vector was used to place a loxP site in the intron downstream of exon 3 and a floxed neomycin-thymidine kinase expression cassette in the upstream intron. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Transient expression from an injected Cre plasmid removed the neomycin-thymidine cassette, but left the floxed exon. Chimeric animals developed from correctly-targeted ES cells were backcrossed to C57BL/6 at least 6 times by the donating laboratory.
|Allele Name||targeted mutation 1, Jamey Marth|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Galnt1, polypeptide N-acetylgalactosaminyltransferase 1|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A loxP site was placed downstream of an exon and a floxed neomycin-thymidine kinase expression cassette was inserted into the upstream intron. Transient expression from an injected Cre plasmid removed the neomycin-thymidine cassette, but left the floxed exon, prior to production of chimeric mice.|
|Mutations Made By|| |
Jamey Marth, Burnham Inst at Univ Calif Santa Barbara
When maintained as a live colony, homozygous crossses may be used.
When using the ppGalNAcT-1F mouse strain in a publication, please cite the originating article(s) and include JAX stock #006895 in your Materials and Methods section.