These floxed mutant mice possess loxP sites flanking exon 7 of the Mgat4 gene. This strain may be useful for generating conditional mutations in applications related to fertilization and embryonic development.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
Mice homozygous for this floxed targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon is deleted by cre expression to produce a null allele.
A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette in intron 6 of the gene and an additional loxP site in intron 7. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with cre expression plasmid to excise the neo fragment and leave a floxed exon 7. Gancyclovir-resistant ES cells were injected into blastocysts. Chimeric animals were crossed with C57BL/6 at least six times by the donating laboratory.
|Allele Name||targeted mutation 1, Jamey Marth|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Mgat4a, mannoside acetylglucosaminyltransferase 4, isoenzyme A|
|Strain of Origin||Not Specified|
|Molecular Note||A targeting vector was designed to flank exon 7 with loxP sites.|
|Mutations Made By|| |
Jamey Marth, Burnham Inst at Univ Calif Santa Barbara
When maintained as a live colony, heterozygous or homozygous crosses may be used.
When using the Mgat4aF mouse strain in a publication, please cite the originating article(s) and include JAX stock #006894 in your Materials and Methods section.