These floxed mutant mice possess loxP sites flanking the single coding exon of the Mgat2 gene. This strain may be useful for generating conditional mutations in applications related to fertilization and embryonic development.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon is deleted by cre expression.
A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of the single coding exon and an additional loxP site upstream of the exon. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl +-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transfected with Cre expression plasmid to excise the neo fragment and leave the floxed gene. Gancyclovir-resistant ES cells were injected into blastocysts. Chimeric animals were crossed with C57BL/6 a minimum of nine times by the donating laboratory.
|Allele Name||targeted mutation 1, Jamey Marth|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Mgat2, mannoside acetylglucosaminyltransferase 2|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||The single coding exon of the endogenous locus was flanked by intronic loxP sites inserted by homologous recombination.|
|Mutations Made By|| |
Jamey Marth, Burnham Inst at Univ Calif Santa Barbara
When maintained as a live colony, homozygous crosses may be used.
When using the Mgat2F mouse strain in a publication, please cite the originating article(s) and include JAX stock #006892 in your Materials and Methods section.