These floxed mutant mice possess loxP sites flanking exons 1 and 2 of the Dpagt1 gene. This strain may be useful for generating conditional mutations in applications related to embryogenesis.
Jamey D Marth, Burnham Inst at Univ Calif Santa Barbara
Mice homozygous for this floxed targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon is deleted by cre expression to produce a null allele.
A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette in the intron downstream of exon 2 and an additional loxP site in the intron upstream of exon 1. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were electroporated with Cre expression plasmid pCre-Hygro to excise the neo fragment and leave floxed exons 1 and 2. Gancyclovir-resistant ES cells were injected into blastocysts. Chimeric animals were crossed with C57BL/6 for at least 6 times by the donating laboratory.
|Allele Name||targeted mutation 2, Jamey Marth|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Dpagt1, dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A single loxP sites was inserted upstream of exon 1 and and additional loxP-flanked nemoycin/thymidine kinase selection cassette was inserted into intron 2. Transient cre expression in ES cells resulted in the deletion of the selection cassette, leaving loxP sites flanking exons 1 and 2.|
|Mutations Made By|| |
Jamey Marth, Burnham Inst at Univ Calif Santa Barbara
When maintained as a live colony, homozygous or heterozygous crosses may be used.
When using the GPTF mouse strain in a publication, please cite the originating article(s) and include JAX stock #006887 in your Materials and Methods section.