B6.129-Gcnt1^tm1Jxm/J

Stock number: 006886
Product name: C2 GlcNAcT KO
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Mice homozygous for this Gcnt1 knock-out exhibit abnormal immune system cell numbers and inflammatory response.

Detailed description

Mice homozygous for the mutation are viable, fertile and do not display any gross physical or behavioral abnormalities. The mice are devoid of significant gene-encoded enzyme activity in spleen, bone marrow, and kidney. They exhibit a moderate increase in the number of neutrophils in the blood, a partial deficiency of selectin ligands, and a mild B cell homing deficit. Neutrophil rolling is reduced on E-, L- and P-selectin substrates.

Development

A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette upstream of the single protein coding exon and a loxP site downstream. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Transient Cre expression in targeted cells excised the neo cassette. Breeding males bearing the floxed exon with ZP3-Cre transgenic females resulted in recombination in oocytes, yielding the Type 1 null allele of this line. The line has been backcrossed more than six times to C57BL/6 by the donating laboratory.

Allele

Symbol: Gcnt1^tm1Jxm
Name: targeted mutation 1, Jamey Marth
MGI accession ID: MGI:2681554
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Gcnt1
Marker name: glucosaminyl (N-acetyl) transferase 1, core 2
Chromosome: 19
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: The single protein coding exon was flanked upstream by a loxP site and downstream by a floxed neomycin resistance cassette. Transient Cre expression in correctly targeted cells resulted in removal of both the coding exon and the neomycin cassette. Enzyme activity was absent in spleen, bone marrow, and kidney tissue of homozygotes.