Mice homozygous for the transgelin knock-in allele (Taglntm2(cre)Yec or commonly referred to as SM22alpha-CreKI) exhibit Cre recombinase activity in adult smooth muscle cells and cardiac myocytes. These mice may be useful for Cre-lox technology applications in studying smooth muscle and cardiac gene function, as well as cardiovascular disease.
Yuqing E Chen, University of Michigan, Medical School
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Tagln | transgelin |
Mice homozygous for this SM22alpha-CreKI allele are viable and fertile. These mice have a Cre-recombinase gene inserted into the endogenous transgelin (SM22alpha) locus. The donating investigator reports that this mutation results in a loss of function of the targeted gene. Cre recombinase activity is shown in adult smooth muscle cells (such as arteries, veins, and visceral organs) and cardiac myocytes, but activity is not observed in the same embryonic tissues. It has been the experience of The Jackson Laboratory that optimal breeding is achieved by mating heterozygous females to homozygous males as female mortality post gestation has been noted in our colony. These SM22alpha-CreKI mice may be useful for Cre-lox technology applications in studying smooth muscle and cardiac gene function, as well as cardiovascular disease.
A targeting vector was designed to insert a Cre-recombinase coding sequence (followed by a PGK-neo cassette) in-frame at the SM22alpha (Tagln or transgelin) initiation site. The construct was electroporated into 129S6/SvEvTac-derived "CMT-1" embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 to generate heterozygotes. This strain was backcrossed to C57BL/6J for at least 12 generations prior to arrival at The Jackson Laboratory. The Y chromosome may not have been fixed to the C57BL/6J genetic background.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | adult smooth muscle cells (such as arteries, veins, and visceral organs) and cardiac myocytes |
Allele Name | targeted mutation 2, Yuqing E Chen |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | SM22alpha-creKI; Taglntm2(cre)Liw |
Gene Symbol and Name | Tagln, transgelin |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | adult smooth muscle cells (such as arteries, veins, and visceral organs) and cardiac myocytes |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 9 |
Molecular Note | The cre recombinase coding sequence was inserted in frame into the initiation site, as well as a PGK-neo. |
Mutations Made By | Jifeng Zhang, University of Michigan, Medical School |
When maintaining a live colony, it has been the experience of The Jackson Laboratory that optimal breeding is achieved by mating heterozygous females to homozygous males. Female mortality post gestation has been noted in our colony.
When using the SM22α-creKI mouse strain in a publication, please cite the originating article(s) and include JAX stock #006878 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Tagln<tm2(cre)Yec> |
Frozen Mouse Embryo | B6.129S6-Tagln<tm2(cre)Yec>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Tagln<tm2(cre)Yec>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Tagln<tm2(cre)Yec>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Tagln<tm2(cre)Yec>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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