These "MIP-GFP" transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the control of the mouse insulin 1 promoter. Fluorescence is detected in pancreatic beta-cells from embryonic day (E)13.5 through adulthood. This mutant mouse strain may be useful in studies of diabetes and pancreatic beta islet cell biology.
Manami Hara, University of Chicago
Genetic Background | Generation |
---|---|
N12F3
|
Allele Type |
---|
Transgenic (Reporter) |
These "MIP-GFP" transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the control of the mouse insulin 1 promoter. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. The fluorescence expression pattern is similar to the patterns seen in other stocks (see Stock No. 006784 and Stock No. 006866). MIP-GFP transgenic mice exhibit normal glucose tolerance and pancreatic insulin levels. The human growth hormone (hGH) sequence in the transgenic insert enhances expression of the EGFP, but hGH is not expressed. This mutant mouse strain may be useful in studies of diabetes and pancreatic beta islet cell biology. Mice homozygous for this "MIP-GFP" transgene are viable and fertile.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct containing the coding region of the EGFP gene and a 2.1kb sequence of the human growth hormone to confer high expression of the EGFP under the control of the mouse insulin 1 gene promoter was injected into fertilized CD1 mouse eggs. Founder line 1 was subsequently established. The transgenic mice were then backcrossed to C57BL/6 for 10 generations (see SNp note below).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Site of Expression | EGFP expression is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. |
Allele Name | transgene insertion 1, Manami Hara |
---|---|
Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | MIP:GFP; MIP-EGFP; MIP-GFP; MIP-GFP (line 1); MIP-GFP (line1); Tg(Ins1-GFP)1Hara |
Gene Symbol and Name | Tg(Ins1-EGFP)1Hara, transgene insertion 1, Manami Hara |
Gene Synonym(s) | |
Promoter | Ins1, insulin I, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP expression is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. |
Strain of Origin | CD-1 |
Chromosome | UN |
Molecular Note | A transgenic construct containing the coding region of the EGFP gene and a 2.1kb sequence of the human growth hormone to confer high expression of the EGFP, under the control of the mouse insulin 1 gene promoter (a 8.5 kb region including -8.5 to +12 bp relative to the transcriptional start site) was injected into fertilized CD1 mouse eggs. Founder line 1 was subsequently established. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. |
Mutations Made By | Manami Hara, University of Chicago |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the MIP-GFP (line 1) mouse strain in a publication, please cite the originating article(s) and include JAX stock #006864 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(Ins1-EGFP)1Hara |
Frozen Mouse Embryo | B6.Cg-Tg(Ins1-EGFP)1Hara/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Ins1-EGFP)1Hara/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Ins1-EGFP)1Hara/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Ins1-EGFP)1Hara/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.