This spontaneous, single point no eyelid mutation (Frem2ne, originally ne) is characterized by eyelid, eye, digit, ear and coat color abnormalities.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Spontaneous | Frem2 | Fras1 related extracellular matrix protein 2 |
Mice homozygous for the no eyelid mutation have a range of phenoytpes with varying penetrance including missing eyelid, closed eyelids, microphthalmia, ocular deformities, malformed digits, malformed ear pinnae, renal agenesis, and slight discoloration of the fur on the head.
View cre expression characterization.
The no eyelids mutation arose spontaneously in 2005 in a mapping cross between STOCK Tg(CAG-Bgeo/GFP)21Lbe/J and CAST/EiJ. A mutant male was found in the F2 generation and was crossed to both a C57BL/6J female and a CAST/EiJ female. No mutants were identified in the offspring of the C57BL/6J female, but the CAST/EiJ female did permit propagation of the mutation and the transgene was selected against to remove it from the mutant stock. The offspring of the CAST/EiJ outcross were intercrossed and the strain has been maintained by sibling intercrossing heterozygotes with homozygotes.
Allele Name | no eyelid |
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Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | Frem2, Fras1 related extracellular matrix protein 2 |
Gene Synonym(s) | |
Strain of Origin | STOCK Tg(CAG-Bgeo/GFP)21Lbe/J |
Chromosome | 3 |
Molecular Note | This spontaneous G-to-A mutation at position 53,547,675 (GRCm38) causes an alanine to valine substitution at residue 2160 in the fourth Calx-beta domain. (NM_172862.3:c.6479C>T, p.Ala2160Val) |
When using the no eyelid mouse strain in a publication, please cite the originating article(s) and include JAX stock #006857 in your Materials and Methods section.
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Heterozygous for ne |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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