| Allele Name | transgene insertion 025, Joseph S Takahashi |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | mPer1::luciferase; mPer1-luc; P1L025 |
| Gene Symbol and Name | Tg(Per1-luc)025Jt, transgene insertion 025, Joseph S Takahashi |
| Gene Synonym(s) | |
| Promoter | Per1, period circadian clock 1, mouse, laboratory |
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| Expressed Gene | luc, luciferase, firefly |
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| Site of Expression | suprachiasmatic nuclei (SCN) with a clear circadian rhythm |
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| Strain of Origin | CD-1 |
| Chromosome | UN |
| General Note | 6 transgenic lines were generated and designated by the authors P1L005 (2 copies/genome), P1L025 (1 copy), P1L095 (3), P1L097 (1), P1L128 (2) and P1L141 (10). Brain sections of mice of line Tg(Per1-luc)025Jt (P1L025) were hybridized with luc and Per1 probes to verify the ability of the promoter to maintain a normal circadian pattern of expression after seven days in constant darkness. As results of other experiments were similar for all six lines, data were not differentiated by line. Mice hemizygous for the transgene express luc mRNA in the suprachiasmatic nuclei (SCN) with a circadian rhythm. SCN slice cultures exhibit a circadian rhythm of luminescence for at least 5 days, peaking each day at 6 hours circadian time. Exposure of mice to light for 6 hours during early subjective night rapidly induces transcription of both luc and endogenous Per1 in the SCN; however, whereas Per1 mRNA levels return to baseline, luc mRNA levels remain high. |
| Molecular Note | A 6,756-base pair DNA fragment containing the putative promoter sequence and the 5'-untranslated region, which encompasses exon 1 and part of exon 2, was ligated to the luciferase gene so that the initiating ATGs of the Per1 and luc genes coincided. |
| Mutations Made By | Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr |
