JAX Mouse Strain Datasheet - 006851

B6.Cg-Tg(Per1-luc)025Jt/J

Stock No:: 006851

Cryo Recovery

Overview

These mPer1-luc transgenc mice may be useful in studying circadian rhythms and as a real-time reporter of Per1 expression.

Donating Investigator

Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr

Genetic overview

Genetic Background	Generation

Tg(Per1-luc)025Jt

Allele Type
Transgenic (Reporter)

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Research Applications

Neurobiology Research
Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Mice hemizygous for the "mPer1-Luc" transgene are viable and fertile, with luciferase (luc) expression driven by the mouse Per1 promoter and 5'-UTR elements. Expression of luc RNA is highly specific to the suprachiasmatic nuclei (SCN) with a clear circadian rhythm that correlates with endogenous Per1. Slice cultures taken from hemizygous mice maintain a circadian rhythm of luminescence for at least 5 days after culturing. A prolonged (6 hour) light pulse treatment during the subjective night rapidly induces both mPer1-luc and endogenous mRNA expression; while the endogenous mRNA level decreased to baseline during the 6 hour period, the mPer1-luc mRNA levels remain higher. These mPer1-luc transgenc mice may be useful in studying circadian rhythms and as a real-time reporter of Per1 expression.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. As the original publication describes mice on a STOCK genetic background, it should be noted that the phenotype could vary from that originally described. The donating investigator reports that no segregating phenotypic traits are observed on the C57BL/6 congenic background. We will modify the strain description if necessary as published results become available.

Development

This "mPer1-luc" transgene was designed with the firefly luciferase gene (luc) downstream of a 6.7 kbp sequence from the period homolog 1 (Per1) promoter and 5'-UTR sequence. This was engineered such that the Per1 initiator ATG coincided with the luciferase initiator ATG. This construct was microinjected into CD1 mouse embryos. Transgenic offspring (founder line P1L025) were found to have approximately 10 tandem repeats at a single integration site. This line was maintained on the CD1 outbred background. After this, transgenic mice were backcrossed to C57BL/6 for 5 generations prior to arriving at The Jackson Laboratory.

Expression Data

Expressed Gene	luc, luciferase, firefly
Site of Expression	suprachiasmatic nuclei (SCN) with a clear circadian rhythm

Control Suggestions

Noncarrier
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Wilsbacher LD; Yamazaki S; Herzog ED; Song EJ; Radcliffe LA; Abe M; Block G; Spitznagel E; Menaker M; Takahashi JS. 2002. Photic and circadian expression of luciferase in mPeriod1-luc transgenic mice invivo. Proc Natl Acad Sci U S A 99(1):489-94. PubMed: 11752392 MGI: J:73700

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Genetics

Tg(Per1-luc)025Jt

Allele Symbol: Tg(Per1-luc)025Jt

Allele Name	transgene insertion 025, Joseph S Takahashi
Allele Type	Transgenic (Reporter)
Allele Synonym(s)	P1L025; mPer1-luc; mPer1::luciferase
Gene Symbol and Name	Tg(Per1-luc)025Jt, transgene insertion 025, Joseph S Takahashi
Gene Synonym(s)	P1L025; mPer1-luc
Promoter	Per1, period circadian clock 1, mouse, laboratory
Expressed Gene	luc, luciferase, firefly
Site of Expression	suprachiasmatic nuclei (SCN) with a clear circadian rhythm
Strain of Origin	CD-1
Chromosome	UN
General Note	6 transgenic lines were generated and designated by the authors P1L005 (2 copies/genome), P1L025 (1 copy), P1L095 (3), P1L097 (1), P1L128 (2) and P1L141 (10). Brain sections of mice of line Tg(Per1-luc)025Jt (P1L025) were hybridized with luc and Per1 probes to verify the ability of the promoter to maintain a normal circadian pattern of expression after seven days in constant darkness. As results of other experiments were similar for all six lines, data were not differentiated by line. Mice hemizygous for the transgene express luc mRNA in the suprachiasmatic nuclei (SCN) with a circadian rhythm. SCN slice cultures exhibit a circadian rhythm of luminescence for at least 5 days, peaking each day at 6 hours circadian time. Exposure of mice to light for 6 hours during early subjective night rapidly induces transcription of both luc and endogenous Per1 in the SCN; however, whereas Per1 mRNA levels return to baseline, luc mRNA levels remain high.
Molecular Note	A 6,756-base pair DNA fragment containing the putative promoter sequence and the 5'-untranslated region, which encompasses exon 1 and part of exon 2, was ligated to the luciferase gene so that the initiating ATGs of the Per1 and luc genes coincided.
Mutations Made By	Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Circadian Rhythms
- Fluorescent protein expression in neural tissue
Research Tools
- Fluorescent Proteins
- Neurobiology Research

References

Wilsbacher LD; Yamazaki S; Herzog ED; Song EJ; Radcliffe LA; Abe M; Block G; Spitznagel E; Menaker M; Takahashi JS. 2002. Photic and circadian expression of luciferase in mPeriod1-luc transgenic mice invivo. Proc Natl Acad Sci U S A 99(1):489-94. PubMed: 11752392 MGI: J:73700

Additional - Tg(Per1-luc)025Jt related

Deery MJ; Maywood ES; Chesham JE; Sladek M; Karp NA; Green EW; Charles PD; Reddy AB; Kyriacou CP; Lilley KS; Hastings MH. 2009. Proteomic analysis reveals the role of synaptic vesicle cycling in sustaining the suprachiasmatic circadian clock. Curr Biol 19(23):2031-6. PubMed: 19913422 MGI: J:156786
Doyle SE; Yoshikawa T; Hillson H; Menaker M. 2008. Retinal pathways influence temporal niche. Proc Natl Acad Sci U S A 105(35):13133-8. PubMed: 18695249 MGI: J:139704

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Please inquire about possible genotypes.

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(Per1-luc)025Jt

Allele Symbol: Tg(Per1-luc)025Jt

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

References

Additional - Tg(Per1-luc)025Jt related

Genotyping Protocols

Breeding Considerations

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability