These floxed mutant mice possess loxP sites flanking exons 3-4 of the Mecp2 gene. This strain may be useful for generating conditional mutations in applications related to neurological and developmental studies of Rett syndrome.
Adrian Bird, University of Edinburgh
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Mecp2 | methyl CpG binding protein 2 |
These mice possess two functional loxP sites flanking exons 3-4 of the targeted gene on the X chromosome. Homozygous females and hemizygous males are viable and fertile. Northern blot analysis showed the expected mature transcript from the Mecp2lox locus. Also detected was an unspliced beta-globin transcript that was introduced into the locus as part of the targeting vector. When these mutant mice are bred to mice that express cre recombinase, resulting offspring will have exons 3-4 deleted in the cre-expressing tissue(s). Mice with this X-linked floxed mutation may be useful in neurological and developmental studies of Rett syndrome.
For example, when crossed to a strain expressing Cre recombinase in nervous tissue (see Stock No. 003771), this mutant mouse strain develops a neurological phenotype that mimics Rett syndrome.
When bred to a strain expressing Cre recombinase in embryonic forebrain GABAergic neurons (see Stock No. 008199 for example), this mutant mouse strain may be useful in studies of diseases related to GABA (gamma-aminobutyric acid)-releasing neurons.
When crossed to a strain expressing Cre recombinase in GABAergic neurons (see Stock No. 017535), these mice exhibit behaviors common to those seen in Rett Syndrome and Autism Spectrum Disorders.
A targeting vector was designed to insert a loxP site upstream of exon 3, as well as a human beta-globin intron 2 and polyadenylation signal followed by a loxp-flanked TK-neo cassette all downstream of the stop codon in exon 4, of the targeted gene. The donating investigator reports that the "middle" loxP site (just 5' of the TK-neo cassette) is non-functional; thus only the loxP sites upstream of exon 3 and downstream of the TK-neo cassette are functional. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and chimeric offspring were bred to C57BL/6 mice. Heterozygous females (with exons 3-4 flanked by functional loxP sites) were mated with wild-type C57BL/6 animals. The resulting hemizygous males were bred with heterozygous females to generate homozygous females. Homozygous females and hemizygous males were bred together for many generations prior to arrival at The Jackson Laboratory.
Allele Name | targeted mutation 1, Adrian Bird |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Mecp2lox |
Gene Symbol and Name | Mecp2, methyl CpG binding protein 2 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | X |
Molecular Note | Insertion of a neomycin resistance cassette into the gene introduced loxP sites that flank exons 3 and 4, and added an intron and polyadenylation signal from the human beta globin gene. From the mutated allele, Northern blot analysis detected the wild type mature transcript and also a transcript in which the beta globin intron was unspliced. |
Mutations Made By | Adrian Bird, University of Edinburgh |
When maintaining a live colony, females homozygous for this X-linked mutation can be bred with males hemizygous for this X-linked mutation.
When using the B6;129P2-Mecp2tm1Bird/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006847 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
X linked -Heterozygous females and Wild-type males for Mecp2<tm1Bird> |
Frozen Mouse Embryo | B6;129P2-Mecp2<tm1Bird>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Mecp2<tm1Bird>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Mecp2<tm1Bird>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Mecp2<tm1Bird>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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