STOCK Lepr^db-9J/Jng

Stock number: 006846
Product name: diabetes(9J)
Research areas: Diabetes and Obesity Research, Reproductive Biology Research

Description

Mice homozygous for this spontaneous leptin receptor mutation exhibit moderate obesity, extreme diabetes, and reduced fecundity.

Detailed description

Mice homozygous for this mutation exhibit moderate obesity and extreme diabetes. The primary investigator reports blood glucose levels reaching 600-850 mg/ml and low plasma insulin levels (1-4 ng/ml). Pancreatic beta cells are small, degranulated and atrophic. The liver is steatotic and strongly PAS-positive for glycogen. Adrenal glands are large with a hyperplastic zona reticularis and appear to be missing the medulla.
Homozygous females have fewer primary oocytes and follicles, as well as a decreased amount of developing and ovulated follicles. Fecundity is decreased resulting in smaller litter sizes and fewer litters.

Development

This spontaneous mutation arose at The Jackson Laboratory on a mixed C57BL/6J, SWR/J and CBA background. The mutation was mapped to Chromosome 4 and complementation testing in the laboratory of Dr. Jurgen Naggert demonstrated that it was a remutation at Lepr^db. The strain was cryopreserved in 2007.

Allele

Symbol: Lepr^db-9J
Name: diabetes 9 Jackson
MGI accession ID: MGI:3770610
Generation method: Spontaneous
Marker symbol: Lepr
Marker name: leptin receptor
Chromosome: 4
Strain of origin: mixed
Molecular note: This mutation was shown to be an allele of the leptin receptor gene by its failure to complement the original diabetes mutation, Lepr^db.
General note: This mutation arose spontaneously in mice of a mixed genetic background (~46% C57BL/6, ~50% SWR, and ~3% CBA estimated from the breeding records).