The transgenic line expresses the mouse NLR family pyrin domain (Nlrp1b). Bone marrow-derived macrophages from these transgenic mice are highly susceptible to necrosis induced by anthrax lethal toxin. This mouse strain may be useful in studies of inflammatory autoimmune and infectious diseases, and of anthrax in particular.
William F Dietrich, Harvard University
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
Mice that are heterozygous or homozygous for the "Nalp1b129S1" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Protein products from both the native and transgenic alleles of the gene are detected by RT-PCR analysis of macrophage-derived mRNA. Bone marrow-derived macrophages from these transgenic mice are highly susceptible to necrosis induced by anthrax lethal toxin, similar to macrophages isolated from inbred 129S1/SvImJ mice, and in contrast to macrophages from inbred C57BL/6J mice, which are resistant. This mouse strain may be useful in studies of inflammatory autoimmune and infectious diseases, and of anthrax in particular.
A 129S1/SvImJ-derived BAC (CT7-336A14) carrying the "Nalp1b129S1" allele was injected into B6xSJL F2 hybrid single-cell embryos. Transgenic founders were identified by PCR using MIT marker D11Die36 and a 129S1 allele-specific marker. The mice were backcrossed to C57BL/6 a total of seven times by the donating investigator.
Expressed Gene | Nlrp1b, NLR family, pyrin domain containing 1B, mouse, laboratory |
---|---|
Site of Expression |
Allele Name | transgene insertion 1, William Dietrich |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Nalp1b129S1; Nlrp1b129S1; Tg(CT7-336A14)1Die |
Gene Symbol and Name | Tg(Nlrp1b)1Die, transgene insertion 1, William Dietrich |
Gene Synonym(s) | |
Promoter | Nlrp1b, NLR family, pyrin domain containing 1B, mouse, laboratory |
Expressed Gene | Nlrp1b, NLR family, pyrin domain containing 1B, mouse, laboratory |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
Molecular Note | The Nlrp1b gene from 129S1/SvImJ mice encoded by a BAC (CT7-336A14, GenBank accession number AC026912) was injected into (C57BL/6J x SJL/J)F2 single cell embryos. |
Mutations Made By | William Dietrich, Harvard University |
When maintained as a live colony, hemizygous or homozygous mice may be crossed.
When using the B6.Cg-Tg(Nlrp1b)1Die/DieJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006840 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Nlrp1b)1Die |
Frozen Mouse Embryo | B6.Cg-Tg(Nlrp1b)1Die/DieJ | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Nlrp1b)1Die/DieJ | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Nlrp1b)1Die/DieJ | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Nlrp1b)1Die/DieJ | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.