These MCK-DGAT2 transgenic mice may be useful in studying lipid accumulation in skeletal muscle, insulin and glucose metabolism, obesity, and type 2 diabetes.
Robert V Farese, Jr., Harvard University School of Public Health
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Mice hemizygous for this MCK-DGAT2 transgene are viable and fertile. Under direction of the mouse muscle creatine kinase (MCK) promoter, these mice overexpress human acyl CoA:diacylglycerol acyltransferase 2 (DGAT2) in striated skeletal muscles, specifically glycolytic (rather than oxidative) muscle. DGAT2 overexpression leads to increased lipid deposition (triacylglycerol, ceramide, and fatty acyl CoA) in glycolytic muscle. This lipid accumulation leads to impaired insulin signaling (insulin resistance), glucose uptake, and glucose tolerance in this tissue, as well as whole-body glucose intolerance. These MCK-DGAT2 transgenic mice may be useful in studying lipid accumulation in skeletal muscle, insulin and glucose metabolism, obesity, and type 2 diabetes.
Of note, these mice may also be useful in conjunction with Liv-DGAT2-low transgenic mice (Stock No. 007744) which exhibit DGAT2 overexpression directed to hepatic tissue.
The MCK-DGAT2 transgene was designed with 4 kb of the mouse muscle creatine kinase (MCK) promoter sequence placed upstream of a 1.2 kb human acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) cDNA sequence, followed by a 2.2 kb human growth hormone minigene (providing the polyA signal). The transgene was microinjected into C57BL/6NHsd fertilized eggs and the resulting transgenic offspring were bred to C57BL/6NHsd. Founder line 10 mice were established and maintained by breeding transgenic mice to wildtype littermates for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred with C57BL/6J to establish the colony.
Expressed Gene | DGAT2, diacylglycerol O-acyltransferase 2, human |
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Site of Expression |
Allele Name | transgene insertion 10, Bob Farese |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | MCK-DGAT2 |
Gene Symbol and Name | Tg(Ckm-DGAT2)10Far, transgene insertion 10, Bob Farese |
Gene Synonym(s) | |
Promoter | Ckm, creatine kinase, muscle, mouse, laboratory |
Expressed Gene | DGAT2, diacylglycerol O-acyltransferase 2, human |
Strain of Origin | C57BL/6NHsd |
Chromosome | UN |
Molecular Note | The targeting construct was designed with 4 kb of the mouse muscle creatine kinase (MCK) promoter sequence placed upstream of a 1.2 kb human acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) cDNA sequence, followed by a 2.2 kb human growth hormone minigene (providing the polyA signal). The transgene was microinjected into C57BL/6NHsd fertilized eggs and the resulting transgenic offspring were bred to C57BL/6NHsd. Founder line 10 mice were established. |
Mutations Made By | Malin Levin, The Sahlgenska Academy, G¿teborg Univ |
When maintaining a live colony, transgenic mice can be bred to wildtype siblings. Alternatively, transgenic mice may be bred together, although a homozygous phenotype is not yet characterized.
When using the C57BL/6-Tg(Ckm-DGAT2)10Far/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006781 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Ckm-DGAT2)10Far |
Frozen Mouse Embryo | C57BL/6-Tg(Ckm-DGAT2)10Far/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Ckm-DGAT2)10Far/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Ckm-DGAT2)10Far/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Ckm-DGAT2)10Far/J | $3373.50 |
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