Overview

Also Known As:FoxP3.GFP, Foxp3-GFP KI, Foxp3^IRES-GFP, Foxp3^EGFP

These "Foxp3EGFP" mice co-express EGFP and the regulatory T cell-specific transcription factor Foxp3 under the control of the endogenous promoter. These mutant mice may be useful in immunological studies, including studies of regulatory T cell proliferation, localization, and antigen independence during the primary immune response.

Donating Investigator

Talal Chatila, Boston Children's Hospital

Genetic overview

Genetic Background	Generation
	N1F3pN2F4 (2020-08-07 00:00:00)

Foxp3^tm2Tch

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter)	Foxp3	forkhead box P3

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Research Applications

Research Tools
Immunology, Inflammation and Autoimmunity Research

View All Research Applications

Base Price

Starting at:

$236.78 Domestic price for female 4-week

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Details

Detailed Description

Homozygous mice are viable and fertile with normal T and B cell development. These "Foxp3EGFP" mice co-express EGFP and the regulatory T cell-specific transcription factor Foxp3 under the control of the endogenous promoter. EGFP expression accurately identifies the Foxp3⁺ T cell population (more than 97% of Foxp3⁺ T cells were EGFP⁺), and Foxp3 mRNA expression strictly segregates with EGFP⁺ T cells. Due to X-inactivation in females, the number of EGFP⁺ CD4⁺ T cells found in the peripheral blood of heterozygous females was approximately half that of hemizygous males. CD4⁺ EGFP⁺ cells also exhibit normal regulatory T cell suppression of effector cell proliferation (following stimulation with anti-CD3 and anti-CD28 monoclonal antibodies). Some EGFP expression is noted in a small population of CD8⁺ thymocytes. These mutant mice may be useful in immunological studies, including studies of regulatory T cell proliferation, localization, and antigen independence during the primary immune response.

This allele is also on a C57BL/6 genetic background (see Stock No. 006772).

Development

A targeting vector was designed to place an IRES-EGFP-SV40 polyA sequence immediately downstream of the endogenous Foxp3 translational stop codon but upstream of the endogenous polyA signal. The construct also introduced a loxP-flanked PGKneo cassette into intron 9. This vector was electroporated into 129X1Sv/J-derived SCC10 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were mated with BALB/c females. The PGKneo cassette was removed by mating founder males with Cre-deleter female mice on a BALB/c congenic background. The resulting mice (now harboring this Foxp3^EGFP allele) were then backcrossed for 8 generations to BALB/cAnNTac inbred mice prior to arrival at The Jackson Laboratory. During the importation process, this strain may have been bred with BALB/cJ inbred mice (Stock No. 000651) for at least one generation.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	EGFP is expressed in the Foxp3⁺ T cell population. Some EGFP expression is noted in a small population of CD8⁺ thymocytes.

Control Suggestions

000651 BALB/cJ

Additional Information

Considerations for Choosing Controls

Selected References

Haribhai D; Lin W; Relland LM; Truong N; Williams CB; Chatila TA. 2007. Regulatory T cells dynamically control the primary immune response to foreign antigen. J Immunol 178(5):2961-72PubMed: 17312141 MGI: J:120713
Lin W; Haribhai D; Relland LM; Truong N; Carlson MR; Williams CB; Chatila TA. 2007. Regulatory T cell development in the absence of functional Foxp3. Nat Immunol 8(4):359-68PubMed: 17273171 MGI: J:120636

View All References

Genetics

Foxp3^tm2Tch

Allele Symbol: Foxp3^tm2Tch

Allele Name	targeted mutation 2, Talal A Chatila
Allele Type	Targeted (Reporter)
Allele Synonym(s)	FoxP3.GFP; Foxp3^EGFP; Foxp3^IRES-GFP; Foxp3^tm2(EGFP)Tch; Foxp3-GFP KI
Gene Symbol and Name	Foxp3, forkhead box P3
Gene Synonym(s)
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	EGFP is expressed in the Foxp3⁺ T cell population. Some EGFP expression is noted in a small population of CD8⁺ thymocytes.
Strain of Origin	129X1/SvJ
Chromosome	X
Molecular Note	An IRES-EGFP-SV40 poly A sequence was inserted immediately downstream of the endogenous Foxp3 translational stop codon, but upstream of the endogenous polyA signal, generating a bicistronic locus encoding both Foxp3 and EGFP. Expression of EGFP is restricted to the T cell lineage, primarily to the CD4+ T cell population.
Mutations Made By	Talal Chatila, Boston Children's Hospital

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T cell specific surface marker
- Fluorescent Proteins
- Genetics Research
  - Tissue/Cell Markers: T cell specific surface marker
  - Tissue/Cell Markers
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - T cell deficiency

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Foxp3^tm2Tch/Foxp3⁺
C.129X1-Foxp3

mammalian phenotype

immune system phenotype
- EGFP expression in cells concordant with Foxp3 expression allows the kinetics of regulatory T cell proliferation and localization during a primary immune response
- females are phenotypically normal and indistinguishable from wild-type littermates

Genotype: Foxp3^tm2Tch/Y
C.129X1-Foxp3

mammalian phenotype

immune system phenotype
- EGFP expression in cells concordant with Foxp3 expression allows the kinetics of regulatory T cell proliferation and localization during a primary immune response
- males are phenotypically normal and indistinguishable from wild-type littermates

References

Haribhai D; Lin W; Relland LM; Truong N; Williams CB; Chatila TA. 2007. Regulatory T cells dynamically control the primary immune response to foreign antigen. J Immunol 178(5):2961-72PubMed: 17312141 MGI: J:120713
Lin W; Haribhai D; Relland LM; Truong N; Carlson MR; Williams CB; Chatila TA. 2007. Regulatory T cell development in the absence of functional Foxp3. Nat Immunol 8(4):359-68PubMed: 17273171 MGI: J:120636

Additional - Foxp3^tm2Tch related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Foxp3
- Standard PCR:Foxp3
- Standard PCR:Foxp3alternate1
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

This mutant allele is located on the X chromosome. When maintaining a live colony, homozygous females are bred with hemizygous males.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Hemizygote
Citation
When using the Foxp3^EGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #006769 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX12 (Maximum)

Pricing & Availability

Available Now

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	X linked - Heterozygous females and Wild-type Males for Foxp3<tm2Tch>, 1 pair minimum

Related Products and Services

Frozen Mouse Embryo	C.Cg-Foxp3<tm2Tch>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	C.Cg-Foxp3<tm2Tch>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	C.Cg-Foxp3<tm2Tch>/J Frozen Embryos	$3373.50
Frozen Mouse Embryo	C.Cg-Foxp3<tm2Tch>/J Frozen Embryos	$3373.50

Payment Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Also Known As:FoxP3.GFP, Foxp3-GFP KI, Foxp3IRES-GFP, Foxp3EGFP

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Foxp3tm2Tch

Allele Symbol: Foxp3tm2Tch

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Foxp3tm2Tch/Foxp3+C.129X1-Foxp3

mammalian phenotype

Genotype: Foxp3tm2Tch/YC.129X1-Foxp3

mammalian phenotype

References

Additional - Foxp3tm2Tch related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As:FoxP3.GFP, Foxp3-GFP KI, Foxp3^IRES-GFP, Foxp3^EGFP

Foxp3^tm2Tch

Allele Symbol: Foxp3^tm2Tch

Genotype: Foxp3^tm2Tch/Foxp3⁺
C.129X1-Foxp3

Genotype: Foxp3^tm2Tch/Y
C.129X1-Foxp3

Additional - Foxp3^tm2Tch related