STOCK Or8a1b^{tm1(Or8a1)Mom} Tg(Or8a1,taulacZ)BMom/MomJ

Stock number: 006741
Product name: M71-TgB M71→M72-IRES-tauGFP
Strain synonyms: M71-TgB x M71->M72-IRES-tauGFP

Development

A taulacZ cassette was inserted downstream of a minigene of 9.2 kb of genomic sequence that included the coding and regulatory sequence Or8a1 (formerly called Olfr151) and the construct was microinjected into (CBA x C57BL/6J)F2 pronuclei. Several founders were obtained and the lines were bred to a strain bearing Or8a1b^{tm1(Or8a1)Mom} (commonly referred to as M71->M72-IRES-tauGFP), which was generated in 129P2/OlaHsd-derived E14 ES cells and outcrossed to C57BL/6J. In 2007 sperm was cryopreserved from males carrying both the transgene and the targeted mutation.

Allele

Symbol: Tg(Or8a1,taulacZ)BMom
Name: transgene insertion B, Peter Mombaerts
MGI accession ID: MGI:3712570
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: M71-TgB; Tg(Olfr151,taulacZ)BMom
Marker symbol: Tg(Or8a1,taulacZ)BMom
Marker name: transgene insertion B, Peter Mombaerts
Marker synonyms: M71-TgB
Chromosome: UN
Strain of origin: Not Applicable
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: GFP is expressed in olfactory sensory neurons that express the olfactory receptor Olfr151. LacZ is expressed in Zone 4 of the olfactory epithelium.
Molecular note: This transgene, generated through pronuclear injection, has 9.2 kb of genomic sequence, with 2.3 kb upstream of the putative transcription start site and 3.8 kb downstream of the coding region, and a taulacZ cassette inserted downstream of the coding sequence.

Allele

Symbol: Or8a1b^{tm1(Or8a1)Mom}
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:3045151
Generation method: Targeted
Synonyms: M71->M72-GFP; M71->M72-IRES-tauGFP; M71-M72; Olfr160^{tm1(Olfr151)Mom}; Olfr160^tm1Mom
Marker symbol: Or8a1b
Marker name: olfactory receptor family 8 subfamily A member 1B
Marker synonyms: GA_x6K02T2PVTD-31389446-31388517; M72; MOR171-3; Olfr160; Olr1194; OR11-318; OST025
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Expressed genes: Or8a1,olfactory receptor family 8 subfamily A member 1,mouse, laboratory; GFP,Green Fluorescent Protein,
Site of expression: GFP is expressed in olfactory sensory neurons.
Molecular note: The entire coding region of Olfr160 was replaced with Olfr151 and an IRES-tauGFP gene introduced via homologous recombination.