Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted | Olfr160 | olfactory receptor 160 |
Allele Type |
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Transgenic (Inserted expressed sequence) |
A taulacZ cassette was inserted downstream of a minigene of 9.2 kb of genomic sequence that included the coding and regulatory sequence Olfr151 and the construct was microinjected into (CBA x C57BL/6J)F2 pronuclei. Several founders were obtained and the lines were bred to a strain bearing Olfr160tm1Mom (commonly referred to as M71->M72-IRES-tauGFP), which was generated in 129P2/OlaHsd-derived E14 ES cells and outcrossed to C57BL/6J. In 2007 sperm was cryopreserved from males carrying both the transgene and the targeted mutation.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | Olfr151, olfactory receptor 151, mouse, laboratory |
Site of Expression | GFP is expressed in olfactory sensory neurons. |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | GFP is expressed in olfactory sensory neurons that express the olfactory receptor Olfr151. LacZ is expressed in Zone 4 of the olfactory epithelium. |
Allele Name | targeted mutation 1, Peter Mombaerts |
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Allele Type | Targeted |
Allele Synonym(s) | M71->M72-GFP; M71->M72-IRES-tauGFP; M71-M72; Olfr160tm1Mom |
Gene Symbol and Name | Olfr160, olfactory receptor 160 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | Olfr151, olfactory receptor 151, mouse, laboratory |
Site of Expression | GFP is expressed in olfactory sensory neurons. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | The entire coding region of Olfr160 was replaced with Olfr151 and an IRES-tauGFP gene introduced via homologous recombination. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
Allele Name | transgene insertion B, Peter Mombaerts |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | M71-TgB |
Gene Symbol and Name | Tg(Olfr151,taulacZ)BMom, transgene insertion B, Peter Mombaerts |
Gene Synonym(s) | |
Promoter | Olfr151, olfactory receptor 151, mouse, laboratory |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | GFP is expressed in olfactory sensory neurons that express the olfactory receptor Olfr151. LacZ is expressed in Zone 4 of the olfactory epithelium. |
Strain of Origin | Not Applicable |
Chromosome | UN |
Molecular Note | This transgene, generated through pronuclear injection, has 9.2 kb of genomic sequence, with 2.3 kb upstream of the putative transcription start site and 3.8 kb downstream of the coding region, and a taulacZ cassette inserted downstream of the coding sequence. |
When using the M71-TgB M71→M72-IRES-tauGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #006741 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Olfr160<tm1Mom> Tg(Olfr151,taulacZ)BMom |
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