Overview

This targeted substitution of the beta 2 adrenergic receptor in place of olfactory receptor 151 is valuable for the study of olfaction, and olfactory sensory neuron signaling, function, and development.

Donating Investigator

Peter Mombaerts, Max Planck Research Unit for Neurogenetics

Genetic overview

Genetic Background	Generation

Olfr151^{tm35(Adrb2)Mom}

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Inserted expressed sequence)	Olfr151	olfactory receptor 151

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Research Applications

Sensorineural Research
Research Tools
Developmental Biology Research
Neurobiology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

This targeted substitution of the beta 2 adrenergic receptor (Adbr2) in place of olfactory receptor 151 (Olfr151, also known as M71) drives expression of this non-olfactory G-protein coupled receptor in a pattern consistent with normal Olfr151 expression as assessed by the bicistronically expressed tauGFP marker. At three weeks of age an average of 1,262 cells in the dorsal main olfactory epithelium express GFP in homozygotes. Co-staining for OMP and GAP43 shows that these olfactory sensory neurons are, as a population, somewhat less mature than wild-type Olfr151-expressing olfactory sensory neurons. These Adbr2-expressing olfactory sensory neurons respond in a dose-dependent manner to isoproterenol, but not to acetophenone, a ligand for Olfr151. Wholemount immunohistochemistry shows that Adbr2 protein localizes in the dendritic cilia of the olfactory sensory neurons, similar to localization of olfactory receptor proteins. When crossed with Olfr151^tm26Mom homozygotes, which have an IRES-taulacZ insertion bicistronically expressed downstream of the endogenous Olfr151 coding sequence (see stock 006676) the compound heterozygous offspring show no double-positive cells. This monoallelic expression proves that allelic exclusion of olfactory receptors does not require the endogenous olfactory receptor protein or DNA sequence. Expression of olfactory receptors and Adbr2 in the main olfactory epithelium, assessed by in situ hybridization, strongly suggests that olfactory sensory neurons that express Adbr2 do not co-express an olfactory receptor, so this substitution permits Adbr2 to function in place of an olfactory receptor with normal monogenic expression and allelic exclusion. In heterozygotes the axons of these Adbr2-expressing olfactory sensory neurons coalesce into homogeneous glomeruli distinct from M71 glomeruli. While there are approximately the same number of M71 glomeruli and Adbr2 glomeruli, the Adbr2 glomeruli are smaller, by approximately half. In compound heterozygotes expressing this allele and Olfr151^{tm14(Adrb2)Mom}, which has the same Adbr2 substitution but with a taulacZ bicistronic marker (see stock 006691), the Adbr2 expressing axons coalesce into the same glomeruli.

Development

The full coding sequence Olfr151 was excised and replaced with the full coding sequence of beta 2 adrenergic receptor, followed by an IRES-tauGFP-ANCF cassette placed 3 bases downstream of the stop codon. This targeting vector was electroporated into 129P2/OlaHsd-derived E14 ES cells in the laboratory of Dr. Peter Mombaerts. Male chimeras were bred to C57BL/6J females and the self-excising neomycin resistance sequence in the ACNf cassette was removed during transmission through the male germline. This segregating B6;129P2 line was maintained by sibling intercrossing and sperm was cryopreserved from homozygous males.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	GFP is expressed in the dorsal main olfactory epithelium.

Selected References

Brennan DJ; Hackethal A; Metcalf AM; Coward J; Ferguson K; Oehler MK; Quinn MA; Janda M; Leung Y; Freemantle M; Webb PM; Spurdle AB; Obermair A. 2014. Serum HE4 as a prognostic marker in endometrial cancer--a population based study. Gynecol Oncol 132(1):159-65PubMed: 24211402 MGI: J:203483

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Genetics

Olfr151^{tm35(Adrb2)Mom}

Allele Symbol: Olfr151^{tm35(Adrb2)Mom}

Allele Name	targeted mutation 35, Peter Mombaerts
Allele Type	Targeted (Reporter, Inserted expressed sequence)
Allele Synonym(s)	beta2AR->M71-IRES-tauGFP
Gene Symbol and Name	Olfr151, olfactory receptor 151
Gene Synonym(s)
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	GFP is expressed in the dorsal main olfactory epithelium.
Strain of Origin	129P2/OlaHsd
Chromosome	9
Molecular Note	The endogenous coding sequence for this olfactory receptor was replaced with the coding sequence for beta 2 adrenergic receptor with an IRES-tauGFP-ANCF cassette inserted 3 nucleotides after the stop codon to yield bicistronic expression of beta 2 adrenergic receptor and GFP in place of this olfactory receptor. The ANCF neomycin selection cassette self-excised during transmission through the male germline leaving a single loxP site.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Olfr151^{tm35(Adrb2)Mom} related

Sensorineural Research
- Olfaction
Research Tools
- Developmental Biology Research
- Sensorineural Research
- Fluorescent Proteins
Developmental Biology Research
Neurobiology Research
- Receptor Defects
  - G protein-coupled receptor

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Olfr151^{tm35(Adrb2)Mom}/Olfr151^{tm35(Adrb2)Mom}
B6;129P2-Olfr151/MomJ

craniofacial phenotype

abnormal olfactory receptor morphology
- in the dorsal main olfactory epithelium, but these respond to isopreoternol, not to acetophenone, and co-staining for OMP and GAP43 indicates that these olfactory sensory neurons are, as a population, somewhat less mature than wild type
- the endogenous olfactory receptor is replaced with the coding sequence for beta 2 adrenergic receptor and the olfactory sensory neurons expressing this receptor form dendritic knobs normally in a similar pattern as those expressing the wild type receptor, in the dorsal main olfactory epithelium, but these respond to isopreoternol, not to acetophenone, and co-staining for OMP and GAP43 indicates that these olfactory sensory neurons are, as a population, somewhat less mature than wild type

taste/olfaction phenotype

abnormal olfactory receptor morphology
- in the dorsal main olfactory epithelium, but these respond to isopreoternol, not to acetophenone, and co-staining for OMP and GAP43 indicates that these olfactory sensory neurons are, as a population, somewhat less mature than wild type
- the endogenous olfactory receptor is replaced with the coding sequence for beta 2 adrenergic receptor and the olfactory sensory neurons expressing this receptor form dendritic knobs normally in a similar pattern as those expressing the wild type receptor, in the dorsal main olfactory epithelium, but these respond to isopreoternol, not to acetophenone, and co-staining for OMP and GAP43 indicates that these olfactory sensory neurons are, as a population, somewhat less mature than wild type

growth/size/body region phenotype

abnormal olfactory receptor morphology
- in the dorsal main olfactory epithelium, but these respond to isopreoternol, not to acetophenone, and co-staining for OMP and GAP43 indicates that these olfactory sensory neurons are, as a population, somewhat less mature than wild type
- the endogenous olfactory receptor is replaced with the coding sequence for beta 2 adrenergic receptor and the olfactory sensory neurons expressing this receptor form dendritic knobs normally in a similar pattern as those expressing the wild type receptor, in the dorsal main olfactory epithelium, but these respond to isopreoternol, not to acetophenone, and co-staining for OMP and GAP43 indicates that these olfactory sensory neurons are, as a population, somewhat less mature than wild type

nervous system phenotype

abnormal olfactory bulb development
- ory sensory neurons that express this receptor in the dorsal main olfactory epithelium is an average of 1,262, which is fewer than the number that normally express the endogenous receptor in wild type mice
- While the axons of the olfactory sensory neurons that express this substituted receptor coalesce normally into the normal number of discrete, homogeneous glomeruli, the size of these glomeruli is approximately half the normal size and the number of olfactory sensory neurons that express this receptor in the dorsal main olfactory epithelium is an average of 1,262, which is fewer than the number that normally express the endogenous receptor in wild type mice

abnormal olfactory receptor morphology
- in the dorsal main olfactory epithelium, but these respond to isopreoternol, not to acetophenone, and co-staining for OMP and GAP43 indicates that these olfactory sensory neurons are, as a population, somewhat less mature than wild type
- the endogenous olfactory receptor is replaced with the coding sequence for beta 2 adrenergic receptor and the olfactory sensory neurons expressing this receptor form dendritic knobs normally in a similar pattern as those expressing the wild type receptor, in the dorsal main olfactory epithelium, but these respond to isopreoternol, not to acetophenone, and co-staining for OMP and GAP43 indicates that these olfactory sensory neurons are, as a population, somewhat less mature than wild type

respiratory system phenotype

abnormal olfactory receptor morphology
- in the dorsal main olfactory epithelium, but these respond to isopreoternol, not to acetophenone, and co-staining for OMP and GAP43 indicates that these olfactory sensory neurons are, as a population, somewhat less mature than wild type
- the endogenous olfactory receptor is replaced with the coding sequence for beta 2 adrenergic receptor and the olfactory sensory neurons expressing this receptor form dendritic knobs normally in a similar pattern as those expressing the wild type receptor, in the dorsal main olfactory epithelium, but these respond to isopreoternol, not to acetophenone, and co-staining for OMP and GAP43 indicates that these olfactory sensory neurons are, as a population, somewhat less mature than wild type

References

Brennan DJ; Hackethal A; Metcalf AM; Coward J; Ferguson K; Oehler MK; Quinn MA; Janda M; Leung Y; Freemantle M; Webb PM; Spurdle AB; Obermair A. 2014. Serum HE4 as a prognostic marker in endometrial cancer--a population based study. Gynecol Oncol 132(1):159-65PubMed: 24211402 MGI: J:203483

Additional References

Bressel OC; Khan M; Mombaerts P. 2016. Linear correlation between the number of olfactory sensory neurons expressing a given mouse odorant receptor gene and the total volume of the corresponding glomeruli in the olfactory bulb. J Comp Neurol 524(1):199-209PubMed: 26100963 MGI: J:221945

Additional - Olfr151^{tm35(Adrb2)Mom} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Olfr151
- Genotyping resources and troubleshooting
Breeding Considerations

This line can be bred to homozygosity since homozygotes are viable and fertile.
- Additional Breeding and Husbandry Support
Citation
When using the B6;129P2-Olfr151^{tm35(Adrb2)Mom}/MomJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006734 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
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Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for Olfr151<tm35(Adrb2)Mom>

Related Products and Services

Frozen Mouse Embryo	B6;129P2-Olfr151<tm35(Adrb2)Mom>/MomJ Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129P2-Olfr151<tm35(Adrb2)Mom>/MomJ Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129P2-Olfr151<tm35(Adrb2)Mom>/MomJ Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6;129P2-Olfr151<tm35(Adrb2)Mom>/MomJ Frozen Embryo	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Selected References

Olfr151tm35(Adrb2)Mom

Allele Symbol: Olfr151tm35(Adrb2)Mom

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Olfr151tm35(Adrb2)Mom related

Mammalian Phenotype Terms by Genotype

Genotype: Olfr151tm35(Adrb2)Mom/Olfr151tm35(Adrb2)MomB6;129P2-Olfr151/MomJ

craniofacial phenotype

taste/olfaction phenotype

growth/size/body region phenotype

nervous system phenotype

respiratory system phenotype

References

Additional References

Additional - Olfr151tm35(Adrb2)Mom related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Olfr151^{tm35(Adrb2)Mom}

Allele Symbol: Olfr151^{tm35(Adrb2)Mom}

Genotype: Olfr151^{tm35(Adrb2)Mom} related

Genotype: Olfr151^{tm35(Adrb2)Mom}/Olfr151^{tm35(Adrb2)Mom}
B6;129P2-Olfr151/MomJ

Additional - Olfr151^{tm35(Adrb2)Mom} related