Transient receptor potential cation channel (Trpc2) is essential in chemosensory signal transduction in mouse vomeronasal sensory neurons, and is also expressed in two types of sensory neurons of the mouse main olfactory epithelium. This knockin allele has an internal ribosome entry site and the microtubule-binding protein tau/beta-galactosidase (taulacZ) fusion protein downstream of the transient receptor potential cation channel (Trpc2) coding sequence to produce bicistronic expression. Vomeronasal and olfactory sensory neurons that express Trpc2 also co-express the taulacZ fusion protein by virtue of IRES-mediated co-translation, permitting single-cell histological assessment of Trpc2 expression. These neurons can be revealed by histochemistry of beta-galactosidase enzymatic activity, or by immunofluorescence with anti-beta-galactosidase antibodies.

This IRES-tau-lacZ knockin allele bicistronically co-expresses tau-lacZ fusion protein with the endogenously expressed transient receptor potential cation channel (Trpc2) and is valuable for expression analysis in the olfactory and vomeronasal systems.

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