This IRES-tau-lacZ knockin allele bicistronically co-expresses tau-lacZ fusion protein with the endogenously expressed transient receptor potential cation channel (Trpc2) and is valuable for expression analysis in the olfactory and vomeronasal systems.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Trpc2 | transient receptor potential cation channel, subfamily C, member 2 |
Transient receptor potential cation channel (Trpc2) is essential in chemosensory signal transduction in mouse vomeronasal sensory neurons, and is also expressed in two types of sensory neurons of the mouse main olfactory epithelium. This knockin allele has an internal ribosome entry site and the microtubule-binding protein tau/beta-galactosidase (taulacZ) fusion protein downstream of the transient receptor potential cation channel (Trpc2) coding sequence to produce bicistronic expression. Vomeronasal and olfactory sensory neurons that express Trpc2 also co-express the taulacZ fusion protein by virtue of IRES-mediated co-translation, permitting single-cell histological assessment of Trpc2 expression. These neurons can be revealed by histochemistry of beta-galactosidase enzymatic activity, or by immunofluorescence with anti-beta-galactosidase antibodies.
The Trpc2tm3Mom mutation was generated in 129P2/OlaHsd derived E14 ES cells and resulting mice were bred to C57BL/6J before being intercrossed to homozygosity. This mutation was generated using the ACNf neo cassette, which self-excises during transmission in the male chimera. In 2007 sperm was cryopreserved from homozygous males on this mixed B6;129P2 background.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | tau-lacZ is expressed in vomeronasal and olfactory sensory neurons. |
Allele Name | targeted mutation 3, Peter Mombaerts |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | Trpc2-IRES-taulacZ |
Gene Symbol and Name | Trpc2, transient receptor potential cation channel, subfamily C, member 2 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | tau-lacZ is expressed in vomeronasal and olfactory sensory neurons. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | An IRES-taulacZ cassette was inserted 32bp downstream of the endogenous coding region so that taulacZ expression is bicistronically expressed to mark endogenous gene expression |
When using the B6;129P2-Trpc2tm3Mom/MomJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006732 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Trpc2<tm3Mom> |
Frozen Mouse Embryo | B6;129P2-Trpc2<tm3Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Trpc2<tm3Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Trpc2<tm3Mom>/MomJ | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Trpc2<tm3Mom>/MomJ | $3373.50 |
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