This strain is valuable for studying vomeronasal sensory neuron signaling, chemodetection, and the role of MHC in pheromone detection.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Del(17)5Mom | deletion, Chr 17, Peter Mombaerts 5 |
Homozygotes are devoid of the nine H2-Mv cluster genes, yet immunohistochemistry does not detect any compensatory increase in expression of MHC class I genes in the basal vomeronasal epithelium. The accessory olfactory bulb appears to develop normally, the tripartite layering of the vemeronasal epithelium is reasonably normal, but there is a minor decrease in the number of basal vomeronasal sensory neurons (VSN) in 10-week-old males. NanoString analysis at 3 weeks of age shows no difference in RNA expression in 37 different vomeronasal receptors, and at 8 weeks of age only a small reduction in expression of 3 out of 36 receptors: Vmn2r10, Vmn2r14, and Vmn2r70. Vmn2r81-expressing VMNs, which normally co-express H2-Mv, have a decreased sensitivity to two separate peptide ligands; Vmn2r26-expressing VMNs, which normally do not co-express H2-Mv, do not show this decreased sensitivity to stimulation. Although Vmn2r81-expressing VMNs show reduced ligand sensitivity, with higher ligand concentration they can still respond at the same maximum level. A diminished sensitivity was also found to stimulation from the high molecular weight fraction of C57BL/6 male urine and to exocrine gland secreted peptide 1. Both homozygous males and homozygous lactating females show decreased aggression toward a castrated intruder male that had been swabbed with high molecular weight fraction of male urine. This diminished aggression can be restored to approximately normal levels by increasing 4 fold the dose of male urine applied to the intruder. Although homozygous males show no change in latency to mount, and homozygous females show no change in duration of investigation of the male anogenital region, homozygous females do display diminished lordosis behavior.
This 532,098 base pair deletion encompasses all nine genes in the H2-Mv cluster. Replacement vectors for each end of the H2-Mv gene cluster were electroporated consecutively into 129S7-derived, Hprt-deficient AB2.2 ES cells. One vector had an Hprt 5prime-loxP-neo cassette and the other had a puro-loxP-Hprt cassette. ES Cell clones with both targeted events were electroporated with the Cre-expression plasmid pOG231, and cultured in hypoxantine-aminopterin-thymidine to select for those clones in which Cre-recombination reconstituted a functional Hprt gene by deleting the large intervening sequence. Selected ES cells were injected into C57BL/6 blastocysts and male chimeras were bred to 129S6/SvEvTac. After several generations of colony expansion, sperm was cryopreserved from homoozygous males. This strain and the C57BL/6J strain derived from it (see stock#008098) were referred to as strain C to distinguish them from strains derived from injecting a different ES cell clone, strains A (see stocks #006730 and #008097).
Allele Name | deletion, Chr 17, Peter Mombaerts 5 |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | deltaH2Mv |
Gene Symbol and Name | Del(17)5Mom, deletion, Chr 17, Peter Mombaerts 5 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 17 |
Molecular Note | Chromosome engineering produced this 532,098 base pair deletion from Chromosome 17 36,283,667bp to 26,815,764bp (GRCm38), and strains were generated from two separate chimeras with Line A assigned this deletion symbol and Line C assigned Del(17)5Mom. |
When using the 129S-Del(17)5Mom/3MomJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006731 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for H2Mv-D, strain C |
Frozen Mouse Embryo | 129S-Del(17)5Mom/3MomJ | $2595.00 |
Frozen Mouse Embryo | 129S-Del(17)5Mom/3MomJ | $2595.00 |
Frozen Mouse Embryo | 129S-Del(17)5Mom/3MomJ | $3373.50 |
Frozen Mouse Embryo | 129S-Del(17)5Mom/3MomJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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