This strain expresses a nuclear localized Cre recombinase in the subset of olfactory sensory neurons that would normally express OLFR124.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
The Olfr124tm5(cre)Mom allele lacks the single exon encoding OLFR124 and instead expresses nuclear localized Cre recombinase driven by the endogenous Olfr124 promoter. This allele has been used for genetic lineage tracing in olfactory sensory neuron development and the consequence of an olfactory receptor null allele where the paradigm of monogenic and monoallelic expression are compromised.
Sequence encoding a nuclear localized Cre recombinase and an ACNF selection cassette was neatly substituted in place of the single endogenous coding exon to yield this null allele that expresses Cre recombinase under the control of the Olfr124 endogenous promoter. The ACNF cassette self-excised during male germline transmission. Homologous recombination was done in 129P2-derived E14 ES cells, the chimeric founder was bred with C57BL/6J and this strain was maintained on a B6;129P2 segregating background until sperm was cryopreserved from homozygous males in 2007.
|Allele Name||targeted mutation 5, Peter Mombaerts|
|Allele Type||Targeted (Recombinase-expressing)|
|Allele Synonym(s)||Cre-SR1; deltaSR1-Cre|
|Gene Symbol and Name||Olfr124, olfactory receptor 124|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||The locus is replaced with a nuclear localized cre. The final allele lacks the self-excising neomycin resistance cassette.|