OR2B4 (formerly OLFR124) expressing olfactory sensory neurons are normally found in the ventral zone of the main olfactory epithelium, densely packed in the septal organ, and OR2B4 expressing axons coalesce into one large glomerulus on the lateral surface of the ventral olfactory bulb and two to four glomeruli clustered in the medial olfactory bulb. OR2B4 expressing cells have been found to respond to a wide array of compounds and at a wide concentration range. The single Or2b4 expressed exon was replaced with a cassette encoding green fluorescent protein so that olfactory sensory neurons with the Or2b4 locus actively transcribed can be identified by their expression of GFP even though no OR2B4 protein is expressed. Olfactory sensory neurons actively transcribing this null reporter allele occupy a more basal position in the main olfactory epithelium and the sensory organ than controls, showing that the migratory pattern is impacted by the alteration in olfactory receptor expression. The number of neurons transcribing this locus in the main olfactory epithelium and especially the sensory organ is significantly reduced compared with OR2B4 expressing cells in mice with an intact Or2b4 locus. Null alleles of Or2b4 permit a very high rate of secondary olfactory receptor expression, with the subset of expressed secondary alleles differing between the main olfactory epithelium and sensory organ.

The single Or2b4 (formerly Olfr124) expressed exon was replaced with a cassette encoding green fluorescent protein so that olfactory sensory neurons that have the Or2b4 locus actively transcribed can be identified by GFP expression, even though no OR2B4 protein is expressed. This null reporter allele is useful in studies to identify olfactory sensory neuronal development, response, and topography in glomeruli in the olfactory bulb.

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