Neuropilin 1 has been found to be important in vasculogenesis, tumerogenesis, axon guidance and neuronal migration. This allele has a loxP flanked exon 2 permitting the generation of a site-specific null when combined with targeted cre expression.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Neuropilin 1 is a cell surface co-receptor for several growth factors and is important in vasculogenesis, tumerogenesis, axon guidance and neuronal migration. This allele has a loxP flanked exon 2. In the absence of cre homozygotes are viable and fertile and without abnormal phenotype. This allele is useful for analyzing the functions of neuropilin 1 through combination with targeted cre expression. Zapiec et al. (2016) used it to characterize the role of neuropilin 1 in olfactory sensory neuron migration and glomeruli positioning in the mouse olfactory bulb.
This allele has a loxP flanked exon 2. It was generated in 129P2/OlaHsd-derived E14 ES Cells and the founder was bred to C57BL/6J. Progeny were subsequently bred to a Tg(ACTB-FLP) deleter, which was initially generated in (C57BL/6 x SJL/J)F2 oocytes, to excise the FRT-flaked neomycin selection cassette. The transgene was bred out and heterozygotes intercrossed to generate homozygotes.
|Allele Name||targeted mutation 1.1, Peter Mombaerts|
|Allele Type||Targeted (Conditional ready (e.g. floxed))|
|Gene Symbol and Name||Nrp1, neuropilin 1|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Exon 2 was flanked with loxP sites and an FRT-flanked neomycin selection cassette and this selection cassette was subsequently excised by crossing to a germline Cre-deleter strain, yielding this conditinal ready allele.|
In the absence of Cre recombinase homozygotes are viable and fertile.
When using the STOCK Nrp1tm1.1Mom/MomJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006707 in your Materials and Methods section.