STOCK Olfr151^{tm14(Adrb2)Mom}/MomJ

Stock number: 006691
Product name: Β2AR→M71-LacZ
Strain synonyms: b2AR->M71-IRES-taulacZ
Research areas: Research Tools, Sensorineural Research

Development

This targeted mutation was generated in 129P2/OlaHsd derived E14 ES cells and the resulting mice were crossed to Tg(EIIa-cre) bearing mice to excise the loxP flanked pgkneo (LNL) cassette. Although this transgene was generated in FVB/N mice, it was backcrossed onto C57BL/6J prior to this cross. The offspring were intercrossed and the transgene was selectively bred out of this line. Thus, this STOCK background is predominantly C57BL/6J and 129P2/OlaHsd. In 2007 sperm were cryopreserved from homozygous males.

The plasmid that was used to generate this mutation is banked and described at Addgene.

Allele

Symbol: Olfr151^{tm14(Adrb2)Mom}
Name: targeted mutation 14, Peter Mombaerts
MGI accession ID: MGI:3047219
Generation method: Targeted
Attributes: Reporter; Inserted expressed sequence
Synonyms: b2AR->M71-IRES-taulacZ; Beta2AR->M71-LacZ
Marker symbol: Olfr151
Marker name: olfactory receptor 151
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Expressed genes: Adrb2,adrenergic receptor, beta 2,mouse, laboratory; lacZ,beta-galactosidase,E. coli
Molecular note: The coding region was replaced with Adrb2 and IRES-taulacZ. Antibody staining indicated Adrb2 expression in cilia, cell bodies and axons of olfactory sensory neurons.