STOCK Or8a1^{tm14(Adrb2)Mom}/MomJ

Stock number: 006691
Product name: Β2AR→M71-LacZ
Strain synonyms: b2AR->M71-IRES-taulacZ
Research areas: Research Tools, Sensorineural Research

Description

This allele has the coding sequence of Or8a1 (formerly called Olfr151 or M71) replaced with the coding sequence for the beta 2 adrenergic receptor (Adrb2) and an IRES-taulacZ reporter.

Detailed description

This allele has the coding sequence of Or8a1 (formerly called Olfr151 or M71) replaced with the coding sequence for the beta 2 adrenergic receptor (Adrb2) and an IRES-taulacZ reporter. Olfactory receptor axons expressing this allele form both medial and lateral glomeruli exclusively and homogeneously, but in a location anterior and ventral to the location usually occupied by Or8a1 expressing axons. Thus, the 7-transmembrane domain receptor Adrb2 sequence was found to functionally substitute for that of an olfactory receptor. See stock No. 006734 for more information.

Development

This targeted mutation was generated in 129P2/OlaHsd derived E14 ES cells and the resulting mice were crossed to Tg(EIIa-cre) bearing mice to excise the loxP flanked pgkneo (LNL) cassette. Although this transgene was generated in FVB/N mice, it was backcrossed onto C57BL/6J prior to this cross. The offspring were intercrossed and the transgene was selectively bred out of this line. Thus, this STOCK background is predominantly C57BL/6J and 129P2/OlaHsd. In 2007 sperm were cryopreserved from homozygous males.

The plasmid that was used to generate this mutation is banked and described at Addgene.

Allele

Symbol: Or8a1^{tm14(Adrb2)Mom}
Name: targeted mutation 14, Peter Mombaerts
MGI accession ID: MGI:3047219
Generation method: Targeted
Attributes: Reporter; Inserted expressed sequence
Marker symbol: Or8a1
Marker name: olfactory receptor family 8 subfamily A member 1
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Expressed genes: Adrb2,adrenergic receptor, beta 2,mouse, laboratory; lacZ,beta-galactosidase,E. coli
Molecular note: The coding region was replaced with Adrb2 and IRES-taulacZ. Antibody staining indicated Adrb2 expression in cilia, cell bodies and axons of olfactory sensory neurons.