Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Inserted expressed sequence) | Olfr151 | olfactory receptor 151 |
This targeted mutation was generated in 129P2/OlaHsd derived E14 ES cells and the resulting mice were crossed to Tg(EIIa-cre) bearing mice to excise the loxP flanked pgkneo (LNL) cassette. Although this transgene was generated in FVB/N mice, it was backcrossed onto C57BL/6J prior to this cross. The offspring were intercrossed and the transgene was selectively bred out of this line. Thus, this STOCK background is predominantly C57BL/6J and 129P2/OlaHsd. In 2007 sperm were cryopreserved from homozygous males.
The plasmid that was used to generate this mutation is banked and described at Addgene.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Expressed Gene | Adrb2, adrenergic receptor, beta 2, mouse, laboratory |
Site of Expression |
Allele Name | targeted mutation 14, Peter Mombaerts |
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Allele Type | Targeted (Reporter, Inserted expressed sequence) |
Allele Synonym(s) | b2AR->M71-IRES-taulacZ; Beta2AR->M71-LacZ |
Gene Symbol and Name | Olfr151, olfactory receptor 151 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Expressed Gene | Adrb2, adrenergic receptor, beta 2, mouse, laboratory |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | The coding region was replaced with Adrb2 and IRES-taulacZ. Antibody staining indicated Adrb2 expression in cilia, cell bodies and axons of olfactory sensory neurons. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
When using the Β2AR→M71-LacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006691 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Olfr151<tm14(Adrb2)Mom> |
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