This allele is useful in studying regulatory expression of olfactory receptor 151. There are five nucleotide substitutions in the promoter that alter both the homeodomain and the O/E-like binding site. Additionally there is a downstream IRES-tauLacZ cassette that is bicistronically expressed.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Olfr151 | olfactory receptor 151 |
This allele of Olfr151 has five nucleotide substitutions in the promoter that alter both the homeodomain and the O/E-like binding site. Additionally there is a downstream IRES-tauLacZ cassette that is bicistronically expresssed. At 26 days of age homozygotes were found to have less than a third the normal number of OLFR151 expressing olfactory sensory neurons and many were located more ventrally in the epithelium than normal. When crossed to a strain with an IRES-tauGFP marker bicistronically expressed with OLFR151 (see Stock # 006676) the compound heterozygote did not show co-expression in the same olfactory sensory neurons so the disruption of the promoter in this allele does not interfere with monoallelic expression.
The Olfr151tm29Mom mutation was generated in 129P2/OlaHsd derived E14 ES cells and resulting mice were bred to C57BL/6J before being intercrossed to homozygosity. This mutation was generated using the ACN neo cassette, which self-excises during transmission in the male chimera. In 2007 sperm was cryopreserved from homozygous males on this mixed B6;129P2 background.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | Homozygotes were found to have a reduced number of labelled olfactory sensory neurons and many were located more ventrally in the epithelium than normal. |
Allele Name | targeted mutation 29, Peter Mombaerts |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | M71 prom Home+O/E-IRES-taulacZ; M71(Hom+O/E)-lacZ |
Gene Symbol and Name | Olfr151, olfactory receptor 151 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | Homozygotes were found to have a reduced number of labelled olfactory sensory neurons and many were located more ventrally in the epithelium than normal. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | Five nucleotide substitutions were made in the promoter, altering both the homeodomain and the O/E-like binding site, and an IRES-taulacZ-ACNf cassette was inserted immediately following the endogenous stop codon. The ACNf cassette self-excised in the male germline leaving no neo sequence. |
Mutations Made By | Peter Mombaerts, Max Planck Research Unit for Neurogenetics |
When using the M71(Hom+O/E)-LacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #006681 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Olfr151<tm29Mom> |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm29Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm29Mom>/MomJ | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm29Mom>/MomJ | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm29Mom>/MomJ | $3373.50 |
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