B6;129P2-Or8a1^tm29Mom/MomJ

Stock number: 006681
Product name: M71(Hom+O/E)-LacZ
Strain synonyms: M71 prom Home+O/E-IRES-taulacZ
Research areas: Neurobiology Research, Research Tools, Sensorineural Research

Description

This allele is useful in studying regulatory expression of olfactory receptor family 8 subfamily A member 1. There are five nucleotide substitutions in the promoter that alter both the homeodomain and the O/E-like binding site. Additionally there is a downstream IRES-tauLacZ cassette that is bicistronically expressed.

Detailed description

This allele of Or8a1 (formerly Olfr151) has five nucleotide substitutions in the promoter that alter both the homeodomain and the O/E-like binding site. Additionally there is a downstream IRES-tauLacZ cassette that is bicistronically expresssed. At 26 days of age homozygotes were found to have less than a third the normal number of OR8A1 expressing olfactory sensory neurons and many were located more ventrally in the epithelium than normal. When crossed to a strain with an IRES-tauGFP marker bicistronically expressed with OR8A1 (see Stock # 006676) the compound heterozygote did not show co-expression in the same olfactory sensory neurons so the disruption of the promoter in this allele does not interfere with monoallelic expression.

Development

The Or8a1^tm29Mom mutation (formerly Olfr151^tm29Mom) was generated in 129P2/OlaHsd derived E14 ES cells and resulting mice were bred to C57BL/6J before being intercrossed to homozygosity. This mutation was generated using the ACN neo cassette, which self-excises during transmission in the male chimera. In 2007 sperm was cryopreserved from homozygous males on this mixed B6;129P2 background.

Allele

Symbol: Or8a1^tm29Mom
Name: targeted mutation 29, Peter Mombaerts
MGI accession ID: MGI:3693508
Generation method: Targeted
Attributes: Reporter
Marker symbol: Or8a1
Marker name: olfactory receptor family 8 subfamily A member 1
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: Homozygotes were found to have a reduced number of labelled olfactory sensory neurons and many were located more ventrally in the epithelium than normal.
Molecular note: Five nucleotide substitutions were made in the promoter, altering both the homeodomain and the O/E-like binding site, and an IRES-taulacZ-ACNf cassette was inserted immediately following the endogenous stop codon. The ACNf cassette self-excised in the male germline leaving no neo sequence.