These GFP/LacZ targeted mutant mice are useful in studies to identify olfactory sensory neuronal development and topography in glomeruli in the olfactory bulb.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Olfr151 | olfactory receptor 151 |
The coding region of the Olfr151 olfactory receptor was replaced by that of the mouse Olfr2 olfactory receptor. Olfactory sensory neurons that express the mutated locus co-express both GFP and taulacZ by virtue of IRES-mediated co-translation. These neurons can be revealed by the intrinsic fluorescence of GFP, by immunofluorescence with anti-GFP antibodies, by histochemistry of beta-galactosidase enzymatic activity, or by immunofluorescence with anti-beta-galactosidase antibodies. Although endogenous Olfr2 is normally expressed in the most ventral zone of the olfactory epithelium, this mutation redirects neurons to the most dorsal zone. These neurons respond to octanal.
The Olfr151tm24(mOlfr2)Mom mutation was generated in 129P2/OlaHsd derived E14 ES cells and the ACNf neomycin resistance cassette was self-excised by cre-recombination in the germline of male chimeras. This line was subsequently bred to C57BL/6J, bred to homozygosity and maintained on a segregating background.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | lacZ, beta-galactosidase, E. coli |
Expressed Gene | Olfr2, olfactory receptor 2, mouse, laboratory |
Site of Expression | GFP is expressed in olfactory sensory neurons. |
Allele Name | targeted mutation 24, Peter Mombaerts |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | mI7->M71-IRES-tauGFP |
Gene Symbol and Name | Olfr151, olfactory receptor 151 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Expressed Gene | Olfr2, olfactory receptor 2, mouse, laboratory |
Site of Expression | GFP is expressed in olfactory sensory neurons. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
When using the mI7→M71-G mouse strain in a publication, please cite the originating article(s) and include JAX stock #006666 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Olfr151<tm24(Olfr2)Mom> |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm24(Olfr2)Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm24(Olfr2)Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm24(Olfr2)Mom>/MomJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Olfr151<tm24(Olfr2)Mom>/MomJ Frozen Embryo | $3373.50 |
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