B6;129P2-Or8a1^{tm24(Or6a2)Mom}/MomJ

Stock number: 006666
Product name: mI7→M71-G
Strain synonyms: mI7->M71-IRES-tauGFP

Description

This olfactory receptor substitution allele is useful in studies to identify olfactory sensory neuronal development, response, and topography in glomeruli in the olfactory bulb.

Detailed description

The coding region of the Or8a1 (formerly Olfr151) olfactory receptor was replaced by that of the mouse Or6a2 (formerly Olfr2) olfactory receptor. Olfactory sensory neurons that express the mutated locus co-express both GFP and taulacZ by virtue of IRES-mediated co-translation. These neurons can be revealed by the intrinsic fluorescence of GFP, by immunofluorescence with anti-GFP antibodies, by histochemistry of beta-galactosidase enzymatic activity, or by immunofluorescence with anti-beta-galactosidase antibodies. Although endogenous Or6a2 is normally expressed in the most ventral zone of the olfactory epithelium, this mutation redirects neurons to the most dorsal zone. These neurons respond to octanal.

Development

The Or8a1^{tm24(Or6a2)Mom} mutation (formerly Olfr151^{tm24(mOlfr2)Mom}) was generated in 129P2/OlaHsd derived E14 ES cells and the ACNf neomycin resistance cassette was self-excised by cre-recombination in the germline of male chimeras. This line was subsequently bred to C57BL/6J, bred to homozygosity and maintained on a segregating background.

Allele

Symbol: Or8a1^{tm24(Or6a2)Mom}
Name: targeted mutation 24, Peter Mombaerts
MGI accession ID: MGI:3692286
Generation method: Targeted
Attributes: Reporter
Marker symbol: Or8a1
Marker name: olfactory receptor family 8 subfamily A member 1
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,; Or6a2,olfactory receptor family 6 subfamily A member 2,mouse, laboratory; lacZ,beta-galactosidase,E. coli
Site of expression: GFP is expressed in olfactory sensory neurons.