FVB-Tg(ACTA1-PABPN1*A17)1Drub/J

Stock number: 006655
Product name: A17-1
Research areas: Neurobiology Research

Description

These mice carry a transgene containing a human skeletal actin (HSA1) promoter upstream of a bovine poly-(A) binding protein nuclear 1 (PABPN1) with 17 alanines. Mice recovered from cryopreserved stock exhibited a loss of expected phenotype (progressive muscle weakness as assessed by locomotor activity and grip strength). A replacement line was obtained from the Donating Investigator, FVB-Tg(ACTA1-PABPN1*A17)1Drub/DrubJ, Stock No. 016193.

Detailed description

These mice carry a transgene containing a human skeletal actin (HSA1) promoter upstream of a bovine poly-(A) binding protein nuclear 1 (PABPN1) with 17 alanines. Mice recovered from cryopreserved stock exhibited a loss of expected phenotype (progressive muscle weakness as assessed by locomotor activity and grip strength). A replacement line was obtained from the Donating Investigator, FVB-Tg(ACTA1-PABPN1*A17)1Drub/DrubJ, Stock No. 016193.

Development

A transgenic construct containing a human skeletal actin (HSA1) promoter upstream of a bovine poly-(A) binding protein nuclear 1 (PABPN1) with 17 alanines was injected into FVB donor eggs.

Allele

Symbol: Tg(ACTA1-PABPN1*A17)1Drub
Name: transgene insertion 1, David Rubinsztein
MGI accession ID: MGI:3693304
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: A17-1; A17.1
Marker symbol: Tg(ACTA1-PABPN1*A17)1Drub
Marker name: transgene insertion 1, David Rubinsztein
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: PABPN1,poly(A) binding protein nuclear 1,human
Molecular note: The transgene contains the human skeletal actin promoter upstream of a mutant bovine cDNA with a series of CGC codons encoding a 17-amino acid polyalanine tract.
General note: Of two lines descended from founders with this transgenic construct, line A7-1 has a more severe phenotype, correlated with higher transgene expression levels, than line A7-2.