Mice hemizygous for this X-linked knock-out mutation lack the normal responsiveness to forskolin and exhibit abnormal projections of axonal OLFR160 olfactory neurons to glomeruli.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Cnga2 | cyclic nucleotide gated channel alpha 2 |
This X-linked mutation disrupts the cAMP-medated olfactory transduction pathway, with the olfactory sensory neurons lacking the normal responsiveness to forskolin. The axonal projections of OLFR160 expressing olfactory neurons project to more glomeruli than normal with a more ventral location than normal, but initial assessment did not reveal abnormal axonal projections for OLFR17 expressing olfactory neurons. Hemizygous males and homozygous females are almost anosmic. Although normal in appearance at birth, most do not survive and usually die within 1 to 2 days of birth because they fail to locate the mother's nipple and suckle. The hemizygous males that are nurtured into adulthood are not good breeders due to lack of sexual and aggressive behavior. Heterozygous females can be used to maintain a colony because they have normal viability and fertility.
This targeted mutation was generated in 129P2/OlaHsd derived E14 embryonic stem cells and the founder chimera was crossed to C57BL/6J. The resulting line was maintained by sibling breeding on a segregating background. Embryos were generated via in vitro fertilization for cryopreservation from heterozygous females and wildtype males.
Allele Name | targeted mutation 1, Peter Mombaerts |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | OCNC1 - |
Gene Symbol and Name | Cnga2, cyclic nucleotide gated channel alpha 2 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | X |
Molecular Note | A neomycin selection cassette replaced sequences corresponding to amino acids 178-678 of the encoded protein. |
Because hemizygous males and homozygous females are nearly anosmic and most die within days of birth due to failure to suckle, but heterozygous females are viable and fertile, heterozygous females can be used as the breeders to maintain this strain. Hemizygotes that are failing to feed can be identified by the absence of a milk spot in the belly and culling non-hemizygotes within the first day may increase the survival of hemizygotes by reducing competition.
When using the OCNC1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006644 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Cnga2<tm1Mom> |
Frozen Mouse Embryo | B6;129P2-Cnga2<tm1Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Cnga2<tm1Mom>/MomJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Cnga2<tm1Mom>/MomJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Cnga2<tm1Mom>/MomJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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