B6;129P2-Cnga2^tm1Mom/MomJ

Stock number: 006644
Product name: OCNC1-
Research areas: Developmental Biology Research, Neurobiology Research, Sensorineural Research

Description

Mice hemizygous for this X-linked knock-out mutation lack the normal responsiveness to forskolin and exhibit abnormal projections of axonal OLFR160 olfactory neurons to glomeruli.

Detailed description

This X-linked mutation disrupts the cAMP-medated olfactory transduction pathway, with the olfactory sensory neurons lacking the normal responsiveness to forskolin. The axonal projections of OLFR160 expressing olfactory neurons project to more glomeruli than normal with a more ventral location than normal, but initial assessment did not reveal abnormal axonal projections for OLFR17 expressing olfactory neurons. Hemizygous males and homozygous females are almost anosmic. Although normal in appearance at birth, most do not survive and usually die within 1 to 2 days of birth because they fail to locate the mother's nipple and suckle. The hemizygous males that are nurtured into adulthood are not good breeders due to lack of sexual and aggressive behavior. Heterozygous females can be used to maintain a colony because they have normal viability and fertility.

Development

This targeted mutation was generated in 129P2/OlaHsd derived E14 embryonic stem cells and the founder chimera was crossed to C57BL/6J. The resulting line was maintained by sibling breeding on a segregating background. Embryos were generated via in vitro fertilization for cryopreservation from heterozygous females and wildtype males.

Allele

Symbol: Cnga2^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:2159016
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cnga2
Marker name: cyclic nucleotide gated channel alpha 2
Chromosome: X
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin selection cassette replaced sequences corresponding to amino acids 178-678 of the encoded protein.