Under the control of a tetracycline-responsive promoter element (tetO) these mice express yellow fluorescent protein (EYFP) specifically in neuronal mitochondria.
Tibor Kristian, University of Maryland
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter, Inducible, Inserted expressed sequence) |
Hemizygotes are viable and fertile. These transgenic mice express a mitochondrial-specific EYFP fusion protein under the control of a tetracycline-responsive promoter element (TRE;tetO). When hemizygotes are bred with another transgenic mouse expressing either reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the regulation of tissue-specific promoter, tissue-specific expression of EYFP in mitochondria of the bitransgenic offspring can be regulated with the tetracycline analog, doxycycline.
When bred to a strain expressing rtTA or tTA in forebrain neurons (see Stock No. 003010 for example), this mutant mouse strain may be useful in studies of neuronal mitochondrial dysfunction.
A transgenic fusion protein construct containing the mitochondrial targeting sequence from the human COX8 gene and Enhanced Yellow Fluorescent Protein (EYFP) was placed downstream of a tet-responsive promoter element (TRE) and upstream of a beta-globin poly A signal. The construct was injected into fertilized C57BL/6 eggs. Founder animals were bred to C57BL/6 for six generations by the donating investigator.
Expressed Gene | COX8A, cytochrome c oxidase subunit 8A, human |
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Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Site of Expression | mitochondrial-specific EYFP fusion protein under the control of a tetracycline-responsive promoter element (TRE or tetO) |
Allele Name | transgene insertion 1, Tibor Kristian |
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Allele Type | Transgenic (Reporter, Inducible, Inserted expressed sequence) |
Allele Synonym(s) | pTRE2-mito/eYFP; Tg(tetO-EYFP-Mito)1Ksn; TRE-mito/EYFP |
Gene Symbol and Name | Tg(tetO-COX8A/EYFP)1Ksn, transgene insertion 1, Tibor Kristian |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | COX8A, cytochrome c oxidase subunit 8A, human |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Site of Expression | mitochondrial-specific EYFP fusion protein under the control of a tetracycline-responsive promoter element (TRE or tetO) |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | A construct encoding a fusion protein comprising the mitochondrial targeting sequence from human cytochrome c oxidase subunit 8A (ubiquitous) joined to enhanced yellow fluorescent protein (EYFP) was placed downstream of the tetracycline responsive element (tetO) and upsteam of a beta-globin polyadenylation signal. A mouse with this transgene can be bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under control of a selected promoter to produce bitransgenic mice in which mitochondrial EYFP expression can be regulated in a tissue-specific manner by administration/removal of the tetracycline analog, doxycycline. |
Mutations Made By | Tibor Kristian, University of Maryland |
When maintained as a live colony, wildtype or C57BL/6J x Hemizygote (or reciprocal) crosses are used.
When using the pTRE-mito/eYFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #006618 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrierfor Tg(tetO-COX8A/EYFP)1Ksn |
Frozen Mouse Embryo | C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J Frozen Embryo | $3373.50 |
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