C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J

Stock number: 006618
Product name: pTRE-mito/eYFP
Research areas: Neurobiology Research, Research Tools

Description

Under the control of a tetracycline-responsive promoter element (tetO) these mice express yellow fluorescent protein (EYFP) specifically in neuronal mitochondria.

Detailed description

Hemizygotes are viable and fertile. These transgenic mice express a mitochondrial-specific EYFP fusion protein under the control of a tetracycline-responsive promoter element (TRE;tetO). When hemizygotes are bred with another transgenic mouse expressing either reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the regulation of tissue-specific promoter, tissue-specific expression of EYFP in mitochondria of the bitransgenic offspring can be regulated with the tetracycline analog, doxycycline.

When bred to a strain expressing rtTA or tTA in forebrain neurons (see Stock No. 003010 for example), this mutant mouse strain may be useful in studies of neuronal mitochondrial dysfunction.

Development

A transgenic fusion protein construct containing the mitochondrial targeting sequence from the human COX8 gene and Enhanced Yellow Fluorescent Protein (EYFP) was placed downstream of a tet-responsive promoter element (TRE) and upstream of a beta-globin poly A signal. The construct was injected into fertilized C57BL/6 eggs. Founder animals were bred to C57BL/6 for six generations by the donating investigator.

Allele

Symbol: Tg(tetO-COX8A/EYFP)1Ksn
Name: transgene insertion 1, Tibor Kristian
MGI accession ID: MGI:3693934
Generation method: Transgenic
Attributes: Reporter; Inducible; Inserted expressed sequence
Marker symbol: Tg(tetO-COX8A/EYFP)1Ksn
Marker name: transgene insertion 1, Tibor Kristian
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: YFP,Yellow Fluorescent Protein,jellyfish; COX8A,cytochrome c oxidase subunit 8A,human
Site of expression: mitochondrial-specific EYFP fusion protein under the control of a tetracycline-responsive promoter element (TRE or tetO)
Molecular note: A construct encoding a fusion protein comprising the mitochondrial targeting sequence from human cytochrome c oxidase subunit 8A (ubiquitous) joined to an enhanced yellow fluorescent protein (EYFP) was placed downstream of the tetracycline responsive element (tetO) and upstream of a beta-globin polyadenylation signal. A mouse with this transgene can be bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under control of a selected promoter to produce bitransgenic mice in which mitochondrial EYFP expression can be regulated in a tissue-specific manner by administration/removal of the tetracycline analog, doxycycline.