NOD.B6-Tg(ML5sHEL)5Ccg/DvsJ

Stock number: 006610
Product name: NOD.sHEL
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

These transgenic mice contain the coding exons of the chicken lysozyme gene (also known as hen egg lysozyme, HEL) linked to a mouse metallothionein I promoter.

Detailed description

Transgenic mice are viable, fertile, normal in size, agouti in color and express a soluble form of hen egg lysozyme. They become diabetic at a rate similar to NOD controls. This strain can be used to study B-cell selection and tolerance as it relates to Type 1 Diabetes.

Development

This transgenic strain was generated by backcrossing transgenic mice on the C57BL/6 background (Stock No. 002599) to NOD/ShiLt for 11 generations. The strain has been maintained subsequently by brother-sister matings. It has been observed that the agouti coat color co-segregates with the transgene; therefore, albino mice are likely to be wild-type and the agouti mice are likely to be transgenic. The Type 1 Diabetes Resource received this stock for Dr. David Serezze's research colony (Stock No. 004252) in 2007 at generation N11F15.

Allele

Symbol: Tg(ML5sHEL)5Ccg
Name: transgene insertion 5, Christopher C Goodnow
MGI accession ID: MGI:2384160
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: MD5; ML5; ML-5; sHEL; solHEL; SWHEL
Marker symbol: Tg(ML5sHEL)5Ccg
Marker name: transgene insertion 5, Christopher C Goodnow
Marker synonyms: MD5; ML5; ML-5; sHEL; solHEL
Chromosome: UN
Strain of origin: C57BL/6JSfd
Expressed genes: HEL,HEL,chicken
Molecular note: A genomic fragment containing the coding exons of the chicken lysozyme gene (also known as hen egg lysozyme, HEL) was linked to a mouse metallothionein I promoter. The mouse metallothionein I promoter is active during embryonic, fetal, and adult life, and can be induced to higher levels of transcription by heavy metals. Mice carrying the transgene had measurable, soluble chicken lysozyme in their serum. The line chosen for study (ML-5) had the highest serum lysozyme concentrations.