These transgenic mice contain the coding exons of the chicken lysozyme gene (also known as hen egg lysozyme, HEL) linked to a mouse metallothionein I promoter.
Dr. David Serreze, The Jackson Laboratory
Genetic Background | Generation |
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001289 NOD/ShiLt |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Transgenic mice are viable, fertile, normal in size, agouti in color and express a soluble form of hen egg lysozyme. They become diabetic at a rate similar to NOD controls. This strain can be used to study B-cell selection and tolerance as it relates to Type 1 Diabetes.
This transgenic strain was generated by backcrossing transgenic mice on the C57BL/6 background (Stock No. 002599) to NOD/ShiLt for 11 generations. The strain has been maintained subsequently by brother-sister matings. It has been observed that the agouti coat color co-segregates with the transgene; therefore, albino mice are likely to be wild-type and the agouti mice are likely to be transgenic. The Type 1 Diabetes Resource received this stock for Dr. David Serezze's research colony (Stock No. 004252) in 2007 at generation N11F15.
Expressed Gene | HEL, HEL, chicken |
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Site of Expression |
Allele Name | transgene insertion 5, Christopher C Goodnow |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | MD5; ML5; ML-5; sHEL; solHEL; SWHEL |
Gene Symbol and Name | Tg(ML5sHEL)5Ccg, transgene insertion 5, Christopher C Goodnow |
Gene Synonym(s) | |
Promoter | Mt1, metallothionein 1, mouse, laboratory |
Expressed Gene | HEL, HEL, chicken |
Strain of Origin | C57BL/6JSfd |
Chromosome | UN |
Molecular Note | A genomic fragment containing the coding exons of the chicken lysozyme gene (also known as hen egg lysozyme, HEL) was linked to a mouse metallothionein I promoter. The mouse metallothionein I promoter is active during embryonic, fetal, and adult life, and can be induced to higher levels of transcription by heavy metals. Mice carrying the transgene had measurable, soluble chicken lysozyme in their serum. The line chosen for study (ML-5) had the highest serum lysozyme concentrations. |
Mutations Made By | Dr. Christopher Goodnow, Stanford University School of Medicine |
When using the NOD.sHEL mouse strain in a publication, please cite the originating article(s) and include JAX stock #006610 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrierfor Tg(ML5sHEL)5Ccg |
Frozen Mouse Embryo | NOD.B6-Tg(ML5sHEL)5Ccg/DvsJ | $2595.00 |
Frozen Mouse Embryo | NOD.B6-Tg(ML5sHEL)5Ccg/DvsJ | $2595.00 |
Frozen Mouse Embryo | NOD.B6-Tg(ML5sHEL)5Ccg/DvsJ | $3373.50 |
Frozen Mouse Embryo | NOD.B6-Tg(ML5sHEL)5Ccg/DvsJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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