Mice of this strain do not express endogenous IgM, and all B lymphocytes express Ig molecules specific for hen egg lyzozyme (HEL). This strain may be used to study defects in B-cell selection as it relates to Type 1 Diabetes.
Dr. David Serreze, The Jackson Laboratory
Genetic Background | Generation |
---|---|
001289 NOD/ShiLt |
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Ighm | immunoglobulin heavy constant mu |
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
NOD.Cg-Ighmtm1Cgn Tg(IghelMD4)4Ccg/DvsJ (NOD.IgHEL Ighm-deficient) mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Because these mice are Ighm-deficient, they do not express endogenous IgM. All B lymphocytes in NOD.IgHEL Ighm-deficient mice express Ig molecules specific for hen egg lyzozyme (HEL). There is no significant difference in the total number of B and T lymphocytes between NOD.IgHEL Ighm-deficient and NOD.IgHEL (Stock No. 006345) mice. The incidence of diabetes in transgenic vs. non-transgenic NOD.Ighm-deficient mice is similar by 21 weeks of age, (16.7% vs. 13.6%, respectively). Histological evaluation of 12 week old NOD.IgHEL Ighm-deficient mice shows that most mice have low levels of insulitis compared to NOD/ShiLt control mice, although an occasional animal had extensive insulitis. In addition, NOD.IgHEL Ighm-deficient mice develop lymphosarcomas after 20 weeks of age. This pathology is not observed in either NOD.Ighm-deficient or NOD.IgHEL transgenic mice.
This strain may be used to study defects in B-cell selection as it relates to Type 1 Diabetes.
NOD.129S2-Ighmtm1Cgn/Dvs (Stock No. 003903) at generation N10 was bred with NOD.B6-Tg(IghelMD4)4Ccg/Dvs (Stock No. 004253). The doubly heterozygous offspring were backcrossed to the former strain (Stock No. 003903) to make the strain homozygous for the Ighmtm1Cgn mutation. Siblings were then intercrossed to make this strain additionally homozygous for Tg(IghelMD4)4Ccg. In 2006, The T1DR received this strain at generation N11F16.
Expressed Gene | Ighg, Immunoglobulin heavy chain (gamma polypeptide), mouse, laboratory |
---|---|
Site of Expression |
Allele Name | targeted mutation 1, University of Cologne |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | B cell-; BCKO; BCR-; BKO; Cmu -; Ig- muMT; Ig-; IgH-; IgHmuMT; Igh-6-; Igh-6null; Igh-6tm1Cgn; Igh-6tm1CgnmuMT0; Ighm-; Igmunull; muIgKO; mum-; muMT; muMt-; mu-MT- |
Gene Symbol and Name | Ighm, immunoglobulin heavy constant mu |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 12 |
Molecular Note | A neomycin resistance cassette disrupted one of the membrane exons of the gene encoding immunoglobulin heavy chain of the class mu (IgM). |
Mutations Made By | Daisuke Kitamura, University of Cologne |
Allele Name | transgene insertion 4, Christopher C Goodnow |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Hel-Ig; HELmuMT; IgHEL; IgTg; IgHEL; Ig-transgenic; MD-3; MD4; MD4 HEL Ig; MD4 Ig; MD4 Ig BCR Tg; MD4-RAG; SWHEL |
Gene Symbol and Name | Tg(IghelMD4)4Ccg, transgene insertion 4, Christopher C Goodnow |
Gene Synonym(s) | |
Promoter | Ighg, Immunoglobulin heavy chain (gamma polypeptide), mouse, laboratory |
Expressed Gene | Ighg, Immunoglobulin heavy chain (gamma polypeptide), mouse, laboratory |
Strain of Origin | C57BL/6 |
Chromosome | 17 |
Molecular Note | The hybridoma HyHEL10 produces an antibody with high affinity for hen egg lysozyme. Genomic clones encoding HyHEL10 immunoglobulin heavy and light chains were used for this construct. The gamma1-constant region of the heavy chain was replaced by a segment containing the mu and delta constant region genes derived from BALB/c mice. The light and heavy chains were co-injected into C57BL/6 eggs. Five lines, MD3-MD7, that carried multiple copies of the transgene construct were created. The MD4 line had the highest expression of the transgene and was propogated for further studies. Mice carrying this transgene had high serum titers of anti-lysozyme antibodies. |
Mutations Made By | Dr. Christopher Goodnow, Stanford University School of Medicine |
Female mice homozygous for the Ighmtm1Cgn mutation and homozygous for the transgene are poor breeders, however males homozygous for the Ighmtm1Cgn mutation and homozygous for the transgene breed well.
When using the NOD.IgHEL,Igh-/- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006608 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Igh-6<tm1Cgn>, Hemizygous or Homozygous for Tg(IghelMD4)4Ccg |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.