These Camkk1 knock-out mice exhibit deficits in conditioned context fear with impaired downstream Camk4 kinase activation. They are suitable for use in applications related to neuronal functions.
Talal Chatila, Boston Children's Hospital
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Camkk1 | calcium/calmodulin-dependent protein kinase kinase 1, alpha |
Homozygotes are viable and fertile. Immunoblots using antibodies against both carboxy- and amino-terminal peptide sequences confirms the absence of wildtype protein in brain tissue. These CaMKKalpha-deficient mice exhibit deficits in context fear during both conditioning and long-term follow-up testing. This is accompanied by impaired activation of other kinases and substrates downstream in the Ca2+/calmodulin kinase (CaMK) cascade. Homozygous mice have normal long-term spatial memory formation, cued fear conditioning, long-term potentiation and anxiety-like behavior levels. These mutant mice may be useful in neurological studies involving the CaMK cascade, neuronal gene transcription, synaptic plasticity, contextual fear and long-term memory consolidation.
A targeting construct was designed to replace exons 2-5 of the targeted gene with a neomycin cassette. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, which were re-implanted in pseudopregnant females. Chimeric males were mated to C57BL/6 females. Mutant mice were then mated to C57BL/6NCrl for 12 generations prior to arrival at The Jackson Laboratory.
Allele Name | targeted mutation 1, Talal A Chatila |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Camkk1, calcium/calmodulin-dependent protein kinase kinase 1, alpha |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 11 |
Molecular Note | A neomycin resistance cassette replaced exons 2 through 5. Immunoblot confirmed absence of protein in mutants. |
Mutations Made By | Talal Chatila, Boston Children's Hospital |
When maintaining a live colony, these mice are bred as homozygotes.
When using the Camkk1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006606 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Camkk1<tm1Tch> |
Frozen Mouse Embryo | B6.129X1-Camkk1<tm1Tch>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Camkk1<tm1Tch>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Camkk1<tm1Tch>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129X1-Camkk1<tm1Tch>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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