These multiple mutant mice express the human histocompatibility complex (MHC) class I HLA-A2.1 allele in the absence of mouse H-2Db, Β2m, and endogenous ecotropic murine leukemia virus (Emv30b).
Dr. David Serreze, The Jackson Laboratory
Genetic Background | Generation |
---|---|
002313 NOD.Cg-Emv30b Prkdcscid/Dvs |
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Prkdc | protein kinase, DNA activated, catalytic polypeptide |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Not Applicable | Emv30 | endogenous ecotropic MuLV 30 |
Allele Type |
---|
Transgenic (Inserted expressed sequence, Humanized sequence) |
Although NOD mice that carry this transgene (Stock No. 006604) develop diabetes significantly earlier than do NOD/ShiLtDvs (NOD) controls, this transgenic strain harbors the Prkdcscid mutation (characterized by an absence of functional T cells and B cells), and therefore does not become diabetic. This strain is useful for adoptive transfer experiments, and as a source of insulitis free pancreatic islets.
This transgene encodes a human B2-microglubulin (B2M) covalently linked to the MHC class 1, alpha1 and alpha2 binding domains of the human HLA-A2.1 gene and the alpha3, cytoplasmic and transmembrane domains of the murine H2-Db protein. The transgene was injected into NOD/ShiLtDvs embryos. Transgenic offspring were mated to NOD.Prkdcscid Emv30b (Stock No. 002313), prior to sibling mating and fixing for homozygosity. In 2007, the T1DR received this stock at generation N2F2.
Expressed Gene | B2M, beta-2-microglobulin, human |
---|---|
Expressed Gene | HLA-A, major histocompatibility complex, class I, A, human |
Site of Expression |
Allele Name | severe combined immunodeficiency |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | SCID |
Gene Symbol and Name | Prkdc, protein kinase, DNA activated, catalytic polypeptide |
Gene Synonym(s) | |
Site of Expression | T and B lymphocytes. |
Strain of Origin | C.BKa-Ighb/Icr |
Chromosome | 16 |
Molecular Note | A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*). |
Allele Name | endogenous ecotropic murine leukemia virus, absent |
---|---|
Allele Type | Not Applicable |
Allele Synonym(s) | null |
Gene Symbol and Name | Emv30, endogenous ecotropic MuLV 30 |
Gene Synonym(s) | |
Strain of Origin | Not Applicable |
Chromosome | 11 |
General Note | The provirus is missing from C57BLKS/J and from the NOR/LtDn stock which was derived from an NOD subline genetically contaminated with C57BLKS/J (J:14300, J:29951). |
Allele Name | transgene insertion 1, David Serreze |
---|---|
Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | HDD; HHD |
Gene Symbol and Name | Tg(HLA-A/H2-D/B2M)1Dvs, transgene insertion 1, David Serreze |
Gene Synonym(s) | |
Promoter | HLA-A2.1, major histocompatibility complex, class I, subtype A2.1, human |
Expressed Gene | B2M, beta-2-microglobulin, human |
Expressed Gene | HLA-A, major histocompatibility complex, class I, A, human |
Strain of Origin | NOD/ShiLtDvs |
Chromosome | UN |
General Note | The HDD construct was created and used to generate a differnet transgenic mouse line in J:41077. |
Molecular Note | This transgene encodes a human B2-microglubulin (B2M) covalently linked to the MHC class 1, alpha1 and alpha2 binding domains of the human HLA-A2.1 gene, and the alpha3, cytoplasmic and transmembrane domains of the murine H2-Db. The construct was injected into fertilized NOD/ShiLtDvs oocytes. The construct was originally described in J:41077 and used to create a different transgenic line. |
Mutations Made By | Dr. David Serreze, The Jackson Laboratory |
When using the NOD.scid HHD mouse strain in a publication, please cite the originating article(s) and include JAX stock #006605 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Prkdc<scid>, Hemizygous or Homozygous for Tg(HLA-A/H2-D/B2M)1Dvs |
Frozen Mouse Embryo | NOD.Cg-Prkdc<scid> Emv30<b> Tg(HLA-A/H2-D/B2M)1Dvs/DvsJ Froz | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Prkdc<scid> Emv30<b> Tg(HLA-A/H2-D/B2M)1Dvs/DvsJ Froz | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Prkdc<scid> Emv30<b> Tg(HLA-A/H2-D/B2M)1Dvs/DvsJ Froz | $3373.50 |
Frozen Mouse Embryo | NOD.Cg-Prkdc<scid> Emv30<b> Tg(HLA-A/H2-D/B2M)1Dvs/DvsJ Froz | $3373.50 |
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