Overview

Also Known As:NOD.scid HHD

These multiple mutant mice express the human histocompatibility complex (MHC) class I HLA-A2.1 allele in the absence of mouse H-2D^b, Β2m, and endogenous ecotropic murine leukemia virus (Emv30^b).

Donating Investigator

Dr. David Serreze, The Jackson Laboratory

Genetic overview

Genetic Background	Generation
002313 NOD.Cg-Emv30^b Prkdc^scid/Dvs

Tg(HLA-A/H2-D/B2M)1Dvs

Allele Type
Transgenic (Inserted expressed sequence, Humanized sequence)

Prkdc^scid

Allele Type	Gene Symbol	Gene Name
Spontaneous	Prkdc	protein kinase, DNA activated, catalytic polypeptide

Emv30^b

Allele Type	Gene Symbol	Gene Name
Not Applicable	Emv30	endogenous ecotropic MuLV 30

View Genetics

Research Applications

Research Tools
Internal/Organ Research
Immunology, Inflammation and Autoimmunity Research
Virology Research
Diabetes and Obesity Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

Although NOD mice that carry this transgene (Stock No. 006604) develop diabetes significantly earlier than do NOD/ShiLtDvs (NOD) controls, this transgenic strain harbors the Prkdc^scid mutation (characterized by an absence of functional T cells and B cells), and therefore does not become diabetic. This strain is useful for adoptive transfer experiments, and as a source of insulitis free pancreatic islets.

Development

This transgene encodes a human B2-microglubulin (B2M) covalently linked to the MHC class 1, alpha1 and alpha2 binding domains of the human HLA-A2.1 gene and the alpha3, cytoplasmic and transmembrane domains of the murine H2-D^b protein. The transgene was injected into NOD/ShiLtDvs embryos. Transgenic offspring were mated to NOD.Prkdc^scid Emv30^b (Stock No. 002313), prior to sibling mating and fixing for homozygosity. In 2007, the T1DR received this stock at generation N2F2.

Expression Data

Expressed Gene	B2M, beta-2-microglobulin, human
Expressed Gene	HLA-A, major histocompatibility complex, class I, A, human
Site of Expression
Site of Expression	T and B lymphocytes.
Site of Expression

Control Suggestions

Additional Information

Considerations for Choosing Controls

Selected References

Pascolo S; Bervas N; Ure JM; Smith AG; Lemonnier FA; Perarnau B. 1997. HLA-A2.1-restricted education and cytolytic activity of CD8(+) T lymphocytes from beta2 microglobulin (beta2m) HLA-A2.1 monochain transgenic H-2Db beta2m double knockout mice. J Exp Med 185(12):2043-51PubMed: 9182675 MGI: J:41077
Takaki T; Marron MP; Mathews CE; Guttmann ST; Bottino R; Trucco M; DiLorenzo TP; Serreze DV. 2006. HLA-A*0201-restricted T cells from humanized NOD mice recognize autoantigens of potential clinical relevance to type 1 diabetes. J Immunol 176(5):3257-65PubMed: 16493087 MGI: J:121685

View All References

Genetics

Tg(HLA-A/H2-D/B2M)1Dvs

Allele Symbol: Tg(HLA-A/H2-D/B2M)1Dvs

Allele Name	transgene insertion 1, David Serreze
Allele Type	Transgenic (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	HDD; HHD
Gene Symbol and Name	Tg(HLA-A/H2-D/B2M)1Dvs, transgene insertion 1, David Serreze
Gene Synonym(s)
Promoter	HLA-A2.1, major histocompatibility complex, class I, subtype A2.1, human
Expressed Gene	B2M, beta-2-microglobulin, human
Expressed Gene	HLA-A, major histocompatibility complex, class I, A, human
Strain of Origin	NOD/ShiLtDvs
Chromosome	UN
General Note	The HDD construct was created and used to generate a differnet transgenic mouse line in J:41077.
Molecular Note	This transgene encodes a human B2-microglubulin (B2M) covalently linked to the MHC class 1, alpha1 and alpha2 binding domains of the human HLA-A2.1 gene, and the alpha3, cytoplasmic and transmembrane domains of the murine H2-D^b. The construct was injected into fertilized NOD/ShiLtDvs oocytes. The construct was originally described in J:41077 and used to create a different transgenic line.
Mutations Made By	Dr. David Serreze, The Jackson Laboratory

Prkdc^scid

Allele Symbol: Prkdc^scid

Allele Name	severe combined immunodeficiency
Allele Type	Spontaneous
Allele Synonym(s)	SCID
Gene Symbol and Name	Prkdc, protein kinase, DNA activated, catalytic polypeptide
Gene Synonym(s)
Site of Expression	T and B lymphocytes.
Strain of Origin	C.BKa-Igh^b/Icr
Chromosome	16
Molecular Note	A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Emv30^b

Allele Symbol: Emv30^b

Allele Name	endogenous ecotropic murine leukemia virus, absent
Allele Type	Not Applicable
Allele Synonym(s)	null
Gene Symbol and Name	Emv30, endogenous ecotropic MuLV 30
Gene Synonym(s)
Strain of Origin	Not Applicable
Chromosome	11
General Note	The provirus is missing from C57BLKS/J and from the NOR/LtDn stock which was derived from an NOD subline genetically contaminated with C57BLKS/J (J:14300, J:29951).

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Prkdc^scid related

Research Tools
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
- Toxicology Research
  - xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B and T cell deficiency
Virology Research
- B and T Cell Deficiency
  - AIDS research tool

Diabetes and Obesity Research
- Type 1 Diabetes (IDDM) Analysis Strains
  - NOD Congenics with Mutations Affecting Immunocompetence
  - NOD Transgenics
- Islet Transplantation Studies
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T cell deficiency, xenograft/transplant host
Immunology, Inflammation and Autoimmunity Research
- HLA transgenics

Mammalian Phenotype Terms by Genotype

References

Pascolo S; Bervas N; Ure JM; Smith AG; Lemonnier FA; Perarnau B. 1997. HLA-A2.1-restricted education and cytolytic activity of CD8(+) T lymphocytes from beta2 microglobulin (beta2m) HLA-A2.1 monochain transgenic H-2Db beta2m double knockout mice. J Exp Med 185(12):2043-51PubMed: 9182675 MGI: J:41077
Takaki T; Marron MP; Mathews CE; Guttmann ST; Bottino R; Trucco M; DiLorenzo TP; Serreze DV. 2006. HLA-A*0201-restricted T cells from humanized NOD mice recognize autoantigens of potential clinical relevance to type 1 diabetes. J Immunol 176(5):3257-65PubMed: 16493087 MGI: J:121685

Additional References

Taylor BA; Rowe L; Grieco DA. 1993. The MEV mouse linkage testing stock: mapping 30 novel proviral insertions and establishment of an improved stock. Genomics 16(2):380-94PubMed: 8390965 MGI: J:4958

Additional - Emv30^b related

Additional - Prkdc^scid related

Additional - Tg(HLA-A/H2-D/B2M)1Dvs related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Appearance
- albino, pink eyed
  Related Genotype: A/A Tyr^c/Tyr^c
Citation
When using the NOD.scid HHD mouse strain in a publication, please cite the originating article(s) and include JAX stock #006605 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous for Prkdc<scid>, Hemizygous or Homozygous for Tg(HLA-A/H2-D/B2M)1Dvs

Related Products and Services

Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> Emv30<b> Tg(HLA-A/H2-D/B2M)1Dvs/DvsJ Froz	$2595.00
Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> Emv30<b> Tg(HLA-A/H2-D/B2M)1Dvs/DvsJ Froz	$2595.00
Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> Emv30<b> Tg(HLA-A/H2-D/B2M)1Dvs/DvsJ Froz	$3373.50
Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> Emv30<b> Tg(HLA-A/H2-D/B2M)1Dvs/DvsJ Froz	$3373.50

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

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Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

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Also Known As:NOD.scid HHD

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(HLA-A/H2-D/B2M)1Dvs

Allele Symbol: Tg(HLA-A/H2-D/B2M)1Dvs

Prkdcscid

Allele Symbol: Prkdcscid

Emv30b

Allele Symbol: Emv30b

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Prkdcscid related

Mammalian Phenotype Terms by Genotype

References

Additional References

Additional - Emv30b related

Additional - Prkdcscid related

Additional - Tg(HLA-A/H2-D/B2M)1Dvs related

Genotyping Protocols

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Prkdc^scid

Allele Symbol: Prkdc^scid

Emv30^b

Allele Symbol: Emv30^b

Genotype: Prkdc^scid related

Additional - Emv30^b related

Additional - Prkdc^scid related