These floxed mutant mice possess loxP sites flanking exons 1 and 2 of the Terf2 gene. This strain may be useful for generating conditional mutations in applications related to telomere structure and function.
Titia de Lange, The Rockefeller University
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Terf2 | telomeric repeat binding factor 2 |
These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele that can result in acute telomere dysfunction.
For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of telomere damage response.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes under the control of the phosphoglyceryl kinase gene promoter was utilized in the construction of this mutant. A loxP site was inserted upstream of exon 1. Another loxP site was inserted downstream of exon 2. This construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into recipient blastocysts. The Donating Investigator maintained the strain on a mixed B6;129P2 background.
Allele Name | targeted mutation 1, Titia de Lange |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | TRF2f |
Gene Symbol and Name | Terf2, telomeric repeat binding factor 2 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 8 |
General Note | After transient Cre expression, homozygous MEFs display a senescence-like arrest of cell proliferation (J:105516). |
Molecular Note | LoxP sites were inserted to flank exons 1 and 2. |
Mutations Made By | Titia de Lange, The Rockefeller University |
When maintaining a live colony, these mice are bred as homozygotes.
When using the TRF2F mouse strain in a publication, please cite the originating article(s) and include JAX stock #006568 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Terf2<tm1Tdl> |
Frozen Mouse Embryo | B6;129P2-Terf2<tm1Tdl>/J | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Terf2<tm1Tdl>/J | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Terf2<tm1Tdl>/J | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Terf2<tm1Tdl>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.