JAX Mouse Strain Datasheet - 006568

B6;129P2-Terf2^tm1Tdl/J

Stock No:: 006568 |; TRF2^F

Targeted Mutation

Cryo Recovery

Overview

Also Known As: TRF2^F

These floxed mutant mice possess loxP sites flanking exons 1 and 2 of the Terf2 gene. This strain may be useful for generating conditional mutations in applications related to telomere structure and function.

Donating Investigator

Titia de Lange, The Rockefeller University

Genetic overview

Genetic Background	Generation

Terf2^tm1Tdl

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Terf2	telomeric repeat binding factor 2

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Research Applications

Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele that can result in acute telomere dysfunction.

For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of telomere damage response.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes under the control of the phosphoglyceryl kinase gene promoter was utilized in the construction of this mutant. A loxP site was inserted upstream of exon 1. Another loxP site was inserted downstream of exon 2. This construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into recipient blastocysts. The Donating Investigator maintained the strain on a mixed B6;129P2 background.

Control Suggestions

Approximate Controls

100492 B6129PF1/J

Additional Information

Considerations for Choosing Controls

Selected References

Celli GB; de Lange T. 2005. DNA processing is not required for ATM-mediated telomere damage response after TRF2 deletion. Nat Cell Biol 7(7):712-8. PubMed: 15968270 MGI: J:105516

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Genetics

Terf2^tm1Tdl

Allele Symbol: Terf2^tm1Tdl

Allele Name	targeted mutation 1, Titia de Lange
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	TRF2^f
Gene Symbol and Name	Terf2, telomeric repeat binding factor 2
Gene Synonym(s)	TRBF2; TRF2
Strain of Origin	129P2/OlaHsd
Chromosome	8
General Note	After transient Cre expression, homozygous MEFs display a senescence-like arrest of cell proliferation (J:105516).
Molecular Note	LoxP sites were inserted to flank exons 1 and 2.
Mutations Made By	Titia de Lange, The Rockefeller University

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - loxP-flanked Sequences

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Terf2^tm1Tdl/Terf2^tm1.1Tdl Tg(Mx1-cre)1Cgn/0
involves: 129/Sv * 129P2/OlaHsd * C57BL/6 * CBA

cellular phenotype

abnormal chromosome morphology
- following pI-pC injection to induce cre expression, telomeres undergo end-to-end fusions in quiescent hepatocytes, indicating telomere deprotection, however, hepatocytes are resistant to this damage and are functional in the presence of chromosome end fusions as indicated by normal liver functionions as indicated by normal liver function
- the telomere fusions seen in mutants do not impair liver regeneration after partial hepatectomy as regeneration occurs without cell division involving endoreduplication and cell growth, circumventing the chromosome segregation problems associated with telomere fusionsomere fusions

liver/biliary system phenotype

abnormal hepatocyte morphology
- following pI-pC injection to induce cre expression, telomeres undergo end-to-end fusions in quiescent hepatocytes, indicating telomere deprotection, however, hepatocytes are resistant to this damage and are functional in the presence of chromosome end fusions as indicated by normal liver functionions as indicated by normal liver function

References

Celli GB; de Lange T. 2005. DNA processing is not required for ATM-mediated telomere damage response after TRF2 deletion. Nat Cell Biol 7(7):712-8. PubMed: 15968270 MGI: J:105516

Additional - Terf2^tm1Tdl related

Bekes M; Okamoto K; Crist SB; Jones MJ; Chapman JR; Brasher BB; Melandri FD; Ueberheide BM; Denchi EL; Huang TT. 2013. DUB-resistant ubiquitin to survey ubiquitination switches in mammalian cells. Cell Rep 5(3):826-38. PubMed: 24210823 MGI: J:205527
Bojovic B; Ho HY; Wu J; Crowe DL. 2013. Stem cell expansion during carcinogenesis in stem cell-depleted conditional telomeric repeat factor 2 null mutant mice. Oncogene 32(43):5156-66. PubMed: 23178498 MGI: J:203237
Celli GB; Denchi EL; de Lange T. 2006. Ku70 stimulates fusion of dysfunctional telomeres yet protects chromosome ends from homologous recombination. Nat Cell Biol 8(8):885-90. PubMed: 16845382 MGI: J:115641
Dimitrova N; Chen YC; Spector DL; de Lange T. 2008. 53BP1 promotes non-homologous end joining of telomeres by increasing chromatin mobility. Nature 456(7221):524-8. PubMed: 18931659 MGI: J:143563
Konishi A; de Lange T. 2008. Cell cycle control of telomere protection and NHEJ revealed by a ts mutation in the DNA-binding domain of TRF2. Genes Dev 22(9):1221-30. PubMed: 18451109 MGI: J:134654
Lazzerini Denchi E; Celli G; de Lange T. 2006. Hepatocytes with extensive telomere deprotection and fusion remain viable and regenerate liver mass through endoreduplication. Genes Dev 20(19):2648-53. PubMed: 17015429 MGI: J:112957
Lottersberger F; Bothmer A; Robbiani DF; Nussenzweig MC; de Lange T. 2013. Role of 53BP1 oligomerization in regulating double-strand break repair. Proc Natl Acad Sci U S A 110(6):2146-51. PubMed: 23345425 MGI: J:194338
Martinez P; Ferrara-Romeo I; Flores JM; Blasco MA. 2014. Essential role for the TRF2 telomere protein in adult skin homeostasis. Aging Cell :. PubMed: 24725274 MGI: J:210514
Peuscher MH; Jacobs JJ. 2011. DNA-damage response and repair activities at uncapped telomeres depend on RNF8. Nat Cell Biol 13(9):1139-45. PubMed: 21857671 MGI: J:176962
Sfeir A; Kabir S; van Overbeek M; Celli GB; de Lange T. 2010. Loss of Rap1 induces telomere recombination in the absence of NHEJ or a DNA damage signal. Science 327(5973):1657-61. PubMed: 20339076 MGI: J:158884
Sfeir A; de Lange T. 2012. Removal of shelterin reveals the telomere end-protection problem. Science 336(6081):593-7. PubMed: 22556254 MGI: J:184522

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Terf2<tm1Tdl>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Also Known As: TRF2F

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Terf2tm1Tdl

Allele Symbol: Terf2tm1Tdl

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Terf2tm1Tdl/Terf2tm1.1Tdl Tg(Mx1-cre)1Cgn/0involves: 129/Sv * 129P2/OlaHsd * C57BL/6 * CBA

cellular phenotype

liver/biliary system phenotype

References

Additional - Terf2tm1Tdl related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: TRF2^F

Terf2^tm1Tdl

Allele Symbol: Terf2^tm1Tdl

Genotype: Terf2^tm1Tdl/Terf2^tm1.1Tdl Tg(Mx1-cre)1Cgn/0
involves: 129/Sv * 129P2/OlaHsd * C57BL/6 * CBA

Additional - Terf2^tm1Tdl related