B6;129P2-Terf2^tm1Tdl/J

Stock number: 006568
Product name: TRF2^F
Research areas: Research Tools

Description

These floxed mutant mice possess loxP sites flanking exons 1 and 2 of the Terf2 gene. This strain may be useful for generating conditional mutations in applications related to telomere structure and function.

Detailed description

These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele that can result in acute telomere dysfunction.

For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of telomere damage response.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes under the control of the phosphoglyceryl kinase gene promoter was utilized in the construction of this mutant. A loxP site was inserted upstream of exon 1. Another loxP site was inserted downstream of exon 2. This construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into recipient blastocysts. The Donating Investigator maintained the strain on a mixed B6;129P2 background.

Allele

Symbol: Terf2^tm1Tdl
Name: targeted mutation 1, Titia de Lange
MGI accession ID: MGI:3619524
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Terf2
Marker name: telomeric repeat binding factor 2
Chromosome: 8
Strain of origin: 129P2/OlaHsd
Molecular note: LoxP sites were inserted to flank exons 1 and 2.
General note: After transient Cre expression, homozygous MEFs display a senescence-like arrest of cell proliferation (J:105516).