MCK-Cre transgenic mice have the Cre recombinase gene driven by the muscle creatine kinase promoter, andCre activity is observed in skeletal and cardiac muscle. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in skeletal and cardiac muscle deletion of the flanked genome.
C. Ronald Kahn, Joslin Diabetes Center
Genetic Background | Generation |
---|---|
N12+N4F2
|
Allele Type |
---|
Transgenic (Recombinase-expressing) |
Starting at:
$255.00 Domestic price for female 4-week |
510.00 Domestic price for breeder pair |
These MCK-Cre transgenic mice have the Cre recombinase gene driven by the muscle creatine kinase (MCK or Ckm) promoter. Cre activity is observed in skeletal and cardiac muscle. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in skeletal and cardiac muscle deletion of the flanked genome.
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Additionally, The Jackson Laboratory colony is viable and fertile as homozygotes.
A transgene was designed with a cre recombinase cDNA sequence (with a SV-40 large T antigen nuclear localization signal and poly(A) signal) inserted in place of the translation initiation site of the Ckm gene. This construct was injected into fertilized FVB embryos which were then implanted into CD1 foster mothers. Chimeric mice were bred to FVB inbred animals to establish transgenic offspring (founder line 5). At some point, mice were bred to insulin receptor mutant mice on a mixed B6;129S4 genetic background. The donating investigator reported that double mutants were backcrossed for 10 generations to C57BL/6 mice (see SNP note below) and then selected for the transgene (and against the targeted mutation) prior to arrival at The Jackson Laboratory.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, at least 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | skeletal and cardiac muscle |
Allele Name | transgene insertion 5, C Ronald Kahn |
---|---|
Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Ckm-cre; Ckmm-cre; Ckmm-NLS-cre; CreMck; mckCRE; MCK-cre; MCKCre+; MCK-cre5; Tg(Ckm-cre)5Khn; Tg(Ckmm-cre)1Khn |
Gene Symbol and Name | Tg(Ckmm-cre)5Khn, transgene insertion 5, C Ronald Kahn |
Gene Synonym(s) | |
Promoter | Ckm, creatine kinase, muscle, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | skeletal and cardiac muscle |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | A 6.5 kb genomic DNA fragment of the Ckmm gene containing the promoter and enhancer 1, untranslated exon 1, 3kb of intron 1 including the enhancer 2 region, and the first 16 bp of exon 2 drives expression of a modified cre with an SV40 large T antigen nuclear localization signal. Expression is directed to the heart and skeletal muscle. |
Mutations Made By | C. Ronald Kahn, Joslin Diabetes Center |
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Additionally, The Jackson Laboratory colony is viable and fertile as homozygotes.
When maintaining a live colony, these mice are bred as hemizygotes or homozygotes.
When using the MCK-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #006475 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Ckmm-cre)5Khn |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.