B6.FVB(129S4)-Tg(Ckmm-cre)5Khn/J

Stock number: 006475
Product name: MCK-Cre
Research areas: Research Tools

Description

MCK-Cre transgenic mice have the Cre recombinase gene driven by the muscle creatine kinase promoter, andCre activity is observed in skeletal and cardiac muscle. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in skeletal and cardiac muscle deletion of the flanked genome.

Detailed description

These MCK-Cre transgenic mice have the Cre recombinase gene driven by the muscle creatine kinase (MCK or Ckm) promoter. Cre activity is observed in skeletal and cardiac muscle. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in skeletal and cardiac muscle deletion of the flanked genome.
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Additionally, The Jackson Laboratory colony is viable and fertile as homozygotes.

Development

A transgene was designed with a cre recombinase cDNA sequence (with a SV-40 large T antigen nuclear localization signal and poly(A) signal) inserted in place of the translation initiation site of the Ckm gene. This construct was injected into fertilized FVB embryos which were then implanted into CD1 foster mothers. Chimeric mice were bred to FVB inbred animals to establish transgenic offspring (founder line 5). At some point, mice were bred to insulin receptor mutant mice on a mixed B6;129S4 genetic background. The donating investigator reported that double mutants were backcrossed for 10 generations to C57BL/6 mice (see SNP note below) and then selected for the transgene (and against the targeted mutation) prior to arrival at The Jackson Laboratory.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, at least 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Tg(Ckmm-cre)5Khn
Name: transgene insertion 5, C Ronald Kahn
MGI accession ID: MGI:2182095
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Ckmm-cre)5Khn
Marker name: transgene insertion 5, C Ronald Kahn
Chromosome: UN
Strain of origin: FVB
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: skeletal and cardiac muscle
Molecular note: A 6.5 kb genomic DNA fragment of the Ckmm gene containing the promoter and enhancer 1, untranslated exon 1, 3kb of intron 1 including the enhancer 2 region, and the first 16 bp of exon 2 drives expression of a modified cre with an SV40 large T antigen nuclear localization signal. Expression is directed to the heart and skeletal muscle.