Homozygous knock-out/reporter mice exhibit altered serum response factor and express lacZ in a pattern corresponding to that of the endogenous Hopx gene with cardiac expression especially prominent.
Rhonda Bassel-Duby, University of Texas Southwestern
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Hopx | HOP homeobox |
Homozygous mice are viable and fertile on this mixed genetic background. Absence of the targeted protein is confirmed in heart and brain tissues from homozygotes. The lacZ expression pattern is similar to that of the endogenous gene. Homozygous heart tissues show altered serum response factor (SRF)-associated gene expression. Mice homozygous for this null allele segregate into two phenotypic classes characterized by an excess or deficiency of cardiac myocytes. These mutant mice may be useful in studying cardiac growth and development.
A targeting vector was designed to replace the entire coding region of the targeted gene with a nuclear lacZ (nLacZ) and neomycin cassette in-frame with the endogenous initiation codon. The construct was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Chimeric animals were crossed with C57BL/6J. Heterozygotes were intercrossed to homozygosity, and then bred as such for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred at least one generation to C57BL/6J to establish the colony.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | The lacZ expression pattern is similar to that of the endogenous Hopx gene. Highest levels of lacZ staining within cardiomyocyte nuclei in the trabecular zone. lacZ staining was also observed within the neural tube. |
Allele Name | targeted mutation 1, Eric N Olson |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | HOP- |
Gene Symbol and Name | Hopx, HOP homeobox |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | The lacZ expression pattern is similar to that of the endogenous Hopx gene. Highest levels of lacZ staining within cardiomyocyte nuclei in the trabecular zone. lacZ staining was also observed within the neural tube. |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 5 |
Molecular Note | The entire coding region was replaced with a cassette containing a nuclear-localized lacZ and neo. Transcript was undetected by Northern blot and RT-PCR analyses of heart and liver tissues from homozygous mutant mice. Western blot analysis confirmed the absence of normal protein in heart and brain tissues. The expression pattern of beta-galactosidase was similar to that of the endogenous gene. |
Mutations Made By | Eric Olson, University of Texas Southwestern Medical |
When maintaining a live colony on this mixed B6;129S genetic background, homozygous mice are bred.
When using the HOP- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006470 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Hopx<tm1Eno> |
Frozen Mouse Embryo | B6;129S-Hopx<tm1Eno>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S-Hopx<tm1Eno>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S-Hopx<tm1Eno>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S-Hopx<tm1Eno>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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