These Chrm1 knock-out mice exhibit elevated dopaminergic transmission.
Dr. Susumu Tonegawa, Massachusetts Institute of Technology
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Chrm1 | cholinergic receptor, muscarinic 1, CNS |
Homozygotes are viable and fertile with complete absence of the wildtype allele mRNA in forebrain tissues. Mice homozygous for this M1 muscarinic acetylcholine receptor deficiency have elevated dopaminergic transmission in the striatum, significantly increased locomotor activity, increased response to the stimulatory effects of amphetamine. These mutant mice may be useful in neurological studies, including the regulation of dopaminergic transmission by the M1 receptor and M1 dysfunction in psychiatric disorders.
A targeting vector was designed to replace a 3kb genomic fragment containing the entire coding sequence of the targeted gene with the neomycin resistance gene. The construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric mice were bred to C57BL/6 mice and then intercrossed to generate mutant mice on a pure C57BL/6 genetic background prior to arrival at The Jackson Laboratory.
Allele Name | targeted mutation 1, Susumu Tonegawa |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | M1 - |
Gene Symbol and Name | Chrm1, cholinergic receptor, muscarinic 1, CNS |
Gene Synonym(s) | |
Strain of Origin | C57BL/6 |
Chromosome | 19 |
Molecular Note | A 3 kb genomic fragment containing the entire coding region of the gene was replaced with a neomycin selection cassette. Northern blot analysis on RNA derived from forebrain of homozygous mice demonstrated that no detectable transcript was produced from this allele. In situ hybridization analysis on sagittal brain sections of homozygous mice further confirmed that the transcript was not expressed. |
Mutations Made By | Dr. Susumu Tonegawa, Massachusetts Institute of Technology |
When maintaining a live colony, homozygous mice are bred.
When using the M1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006468 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Chrm1<tm1Stl> |
Frozen Mouse Embryo | C57BL/6-Chrm1<tm1Stl>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Chrm1<tm1Stl>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Chrm1<tm1Stl>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Chrm1<tm1Stl>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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