| Allele Name | transgene insertion 330, Allan Basbaum |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | Tg(ACTB-lacZ-WGA)330Bbm; Tg(CAG-lacZ-WGA)330Bbm; ZW; ZW19 |
| Gene Symbol and Name | Tg(CAG-lacZ-WGA)330Bbm, transgene insertion 330, Allan Basbaum |
| Gene Synonym(s) | |
| Promoter | ACTB, actin, beta, chicken |
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| Expressed Gene | WGA, Wheat Germ Agglutin, truncated, |
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| Expressed Gene | lacZ, beta-galactosidase, E. coli |
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| Site of Expression | lacZ is expressed throughout central and peripheral nervous system; when crossed with a cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin expression |
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| Strain of Origin | (C57BL/6J x CBA/J)F1 |
| Chromosome | UN |
| General Note | Of three transgenic lines generated - called ZW5, ZW16 and ZW19 by Braz and colleagues (2002) - only ZW19, which exhibited the highest transmission rates, was propagated. |
| Molecular Note | The transgene is comprised of a minimal immediate-early CMV enhancer/chicken beta-actin promoter driving ubiquitous expression of a lox-P flanked beta-galactosidase/neomycin phosphotransferase II (beta-geo) fusion gene that terminates in a strong, triple polyadenylation signal, followed by a C-terminally truncated wheat germ agglutinin coding sequence. Neuron-specific excision of the beta-geo gene by cre recombinase permits expression of the lectin, whose migration allows tracing of synapses involving the expressing cells. |
| Mutations Made By | Joao Braz, University of California San Francisco |
