These ZW mice harbor a Cre-conditional pCZW transgene and have widespread expression of lacZ under the control of the CMV enhancer/chicken actin promoter. When bred to mice expressing Cre recombinase, the "floxed" lacZ sequence is removed in the resulting offspring, and lacZ expression is replaced by wheat germ agglutinin (plant lectin) expression in all cre-expressing cells. These ZW mice may be useful for global expression of lacZ or, when crossed to a cre-expressing strain, for tracing transneuronal or trans-synaptic connections and circuits in brain regions or in the spinal cord.
Allan Basbaum, Univ. California, San Francisco
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
It is reported that some animals genotyping positive for the transgene may not express the transgene; it is strongly recommended to also test animals by lacZ staining of ear notches.
These ZW transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread, but mosaic, throughout the central and peripheral nervous systems. Purkinje cells display intense beta-galactosidase activity. Approximately 50% of the total neuron population express the transgene, as detected by beta-galactosidase activity. Newborn mice exhibit widespread beta-galactosidase activity. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin (plant lectin) expression in tissues expressing cre. The double reporter system makes it possible to distinguish a lack of reporter (lacZ) expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity at the individual cell level. This transgenic mouse strain may be useful in tracing transneuronal or trans-synaptic connections and circuits in brain regions or in the spinal cord. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
A transgenic construct containing sequence encoding a truncated wheat germ agglutinin (lacking the C-terminal) downstream of the chicken beta-actin promoter and a loxP flanked lacZ (beta-geo/3xpA) encoding sequence, was injected into the pronuclei of B6CBAF1/J donor eggs. The resulting transgenic animals were interbred.
Expressed Gene | WGA, Wheat Germ Agglutin, truncated, |
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Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ is expressed throughout central and peripheral nervous system; when crossed with a cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin expression |
Allele Name | transgene insertion 330, Allan Basbaum |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Tg(ACTB-lacZ-WGA)330Bbm; Tg(CAG-lacZ-WGA)330Bbm; ZW; ZW19 |
Gene Symbol and Name | Tg(CAG-lacZ-WGA)330Bbm, transgene insertion 330, Allan Basbaum |
Gene Synonym(s) | |
Promoter | ACTB, actin, beta, chicken |
Expressed Gene | WGA, Wheat Germ Agglutin, truncated, |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ is expressed throughout central and peripheral nervous system; when crossed with a cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin expression |
Strain of Origin | (C57BL/6J x CBA/J)F1 |
Chromosome | UN |
General Note | Of three transgenic lines generated - called ZW5, ZW16 and ZW19 by Braz and colleagues (2002) - only ZW19, which exhibited the highest transmission rates, was propagated. |
Molecular Note | The transgene is comprised of a minimal immediate-early CMV enhancer/chicken beta-actin promoter driving ubiquitous expression of a lox-P flanked beta-galactosidase/neomycin phosphotransferase II (beta-geo) fusion gene that terminates in a strong, triple polyadenylation signal, followed by a C-terminally truncated wheat germ agglutinin coding sequence. Neuron-specific excision of the beta-geo gene by cre recombinase permits expression of the lectin, whose migration allows tracing of synapses involving the expressing cells. |
Mutations Made By | Joao Braz, University of California San Francisco |
When maintaining a live colony, hemizygous mice are bred to wildtype siblings or to B6CBAF1/J (Stock No. 100011).
It is reported that some animals genotyping positive for the transgene may not express the transgene; it is strongly recommended to also test animals by lacZ staining of ear notches.
When using the B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006465 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(CAG-lacZ-WGA)330Bbm |
Frozen Mouse Embryo | B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J Frozen Embryo | $3373.50 |
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