Overview

Also Known As: Tmprss13-

LacZ expression in these Tmprss13 knock-out/reporter mice is detected particularly in the epidermis and urinary bladder

Genetic overview

Genetic Background	Generation

Tmprss13^tm1Dgen

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Tmprss13	transmembrane protease, serine 13

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Research Applications

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression

Selected References

Deltagen Inc. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data SubmissionMGI: J:101679

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Genetics

Tmprss13^tm1Dgen

Allele Symbol: Tmprss13^tm1Dgen

Allele Name	targeted mutation 1, Deltagen
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	targeted mutation 1, Deltagen; Tmprss13^tm1Dgen
Gene Symbol and Name	Tmprss13, transmembrane protease, serine 13
Gene Synonym(s)	MSP; MSPL; MSPS; cDNA sequence BC010843; TMPRSS11; RGD1310872; BC010843
Expressed Gene	lacZ, beta-galactosidase, E. coli
Strain of Origin	129P2/OlaHsd
Chromosome	9
Molecular Note	A 4 kb region, including the last 92 bp of exon 9 and the entire exon 10, was replaced with a promoterless LacZ-Neo fusion gene (beta-galactosidase reporter). This deletion includes exon 9 sequences that encode Asp395, which forms part of the catalytic His-Asp-Ser triad. The in-frame insertion of the LacZ-Neo fusion gene leads to the expression of a fusion protein consisting of the first 377 amino acids fused to beta-galactosidase. High and moderate levels of RNA transcripts were detected in urinary bladder, skin, tongue, seminal vesicle, coagulating gland, and prostate gland. No RNA transcripts are detectable in nervous tissue, liver, bone marrow, small intestine, or cecum. LacZ expression was detected in many tissues examined, particularly in the epidermis and urinary bladder. No LacZ expression was detected in spinal cord, sciatic nerve, spleen, lymph nodes, bone marrow, aorta, liver, pancreas, pituitary gland, adrenal gland, skeletal muscle, testis, ovary, or uterus. Detailed X-gal staining analysis of young adult heterozygotes revealed particularly strong expression in multi-layered squamous epithelium, including the epidermis, hair follicles, oral epithelium, cornea and upper digestive tract, high levels of expression in the transitional epithelium of the bladder, and much lower levels in the prostate, heart, intestine, kidney and thymus.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tmprss13^tm1Dgen/Tmprss13^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6

normal phenotype

no abnormal phenotype detected

Genotype: Tmprss13^tm1Dgen/Tmprss13^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6J

homeostasis/metabolism phenotype

impaired skin barrier function
- Normal - however, no differences in birth weight, postnatal or long term survival are observed
- newborn homozygotes show a 54% increase in the transepidermal fluid loss rate relative to wild-type controls

integument phenotype

abnormal epidermis stratum corneum morphology
- however, no abnormalities are observed in the adult stratum corneum
- newborn homozygotes show an abnormally compacted stratum corneum, with fewer and smaller intercorneocyte lacunae than wild-type controls, indicating a defect in stratum corneum maturation
- the thickness of neonatal stratum corneum is reduced by 38%, whereas the thickness of the living (basal, spinous and granular/transitional) layer of the epidermis is normal

impaired skin barrier function
- Normal - however, no differences in birth weight, postnatal or long term survival are observed
- newborn homozygotes show a 54% increase in the transepidermal fluid loss rate relative to wild-type controls

short vibrissae
- at birth, homozygotes show a 30% reduction in whisker length relative to wild-type controls; however, this difference is transient, as normal size whiskers are observed in adulthood

mammalian phenotype

homeostasis/metabolism phenotype
- Normal - blood chemistry analysis revealed no significant changes in the serum levels of alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, amylase, and creatine kinase, and only a moderate increase in lactate dehydrogenase levels

integument phenotype
- Normal - no abnormalities in pelage hair eruption or pelage hair appearance are observed
- Normal - surprisingly, newborn homozygotes display normal tight junction formation and profilaggrin processing in the epidermis

References

Deltagen Inc. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data SubmissionMGI: J:101679

Additional - Tmprss13^tm1Dgen related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Tmprss13^tm1Dgen
- Genotyping resources and troubleshooting
Citation
When using the Tmprss13- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006434 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wild-type for Tmprss13<tm1Dgen>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: Tmprss13-

Genetic overview

Research Applications

Base Price

Detailed Description

Expression Data

Selected References

Tmprss13tm1Dgen

Allele Symbol: Tmprss13tm1Dgen

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tmprss13tm1Dgen/Tmprss13tm1Dgeninvolves: 129P2/OlaHsd * C57BL/6

normal phenotype

Genotype: Tmprss13tm1Dgen/Tmprss13tm1Dgeninvolves: 129P2/OlaHsd * C57BL/6J

homeostasis/metabolism phenotype

integument phenotype

mammalian phenotype

References

Additional - Tmprss13tm1Dgen related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Tmprss13^tm1Dgen

Allele Symbol: Tmprss13^tm1Dgen

Genotype: Tmprss13^tm1Dgen/Tmprss13^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6

Genotype: Tmprss13^tm1Dgen/Tmprss13^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6J

Additional - Tmprss13^tm1Dgen related