Overview

Also Known As:Tmprss13-

LacZ expression in these Tmprss13 knock-out/reporter mice is detected particularly in the epidermis and urinary bladder

Genetic overview

Genetic Background	Generation

Tmprss13^tm1Dgen

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Tmprss13	transmembrane protease, serine 13

View Genetics

Research Applications

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression

Selected References

Deltagen Inc. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data SubmissionMGI: J:101679

View All References

Genetics

Tmprss13^tm1Dgen

Allele Symbol: Tmprss13^tm1Dgen

Allele Name	targeted mutation 1, Deltagen
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	Tmprss13^-
Gene Symbol and Name	Tmprss13, transmembrane protease, serine 13
Gene Synonym(s)
Expressed Gene	lacZ, beta-galactosidase, E. coli
Strain of Origin	129P2/OlaHsd
Chromosome	9
Molecular Note	A 4 kb region, including the last 92 bp of exon 9 and the entire exon 10, was replaced with a promoterless LacZ-Neo fusion gene (beta-galactosidase reporter). This deletion includes exon 9 sequences that encode Asp395, which forms part of the catalytic His-Asp-Ser triad. The in-frame insertion of the LacZ-Neo fusion gene leads to the expression of a fusion protein consisting of the first 377 amino acids fused to beta-galactosidase. High and moderate levels of RNA transcripts were detected in urinary bladder, skin, tongue, seminal vesicle, coagulating gland, and prostate gland. No RNA transcripts are detectable in nervous tissue, liver, bone marrow, small intestine, or cecum. LacZ expression was detected in many tissues examined, particularly in the epidermis and urinary bladder. No LacZ expression was detected in spinal cord, sciatic nerve, spleen, lymph nodes, bone marrow, aorta, liver, pancreas, pituitary gland, adrenal gland, skeletal muscle, testis, ovary, or uterus. Detailed X-gal staining analysis of young adult heterozygotes revealed particularly strong expression in multi-layered squamous epithelium, including the epidermis, hair follicles, oral epithelium, cornea and upper digestive tract, high levels of expression in the transitional epithelium of the bladder, and much lower levels in the prostate, heart, intestine, kidney and thymus.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tmprss13^tm1Dgen/Tmprss13^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6

normal phenotype

no abnormal phenotype detected

Genotype: Tmprss13^tm1Dgen/Tmprss13^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6J

homeostasis/metabolism phenotype

impaired skin barrier function
- Normal - however, no differences in birth weight, postnatal or long term survival are observed
- newborn homozygotes show a 54% increase in the transepidermal fluid loss rate relative to wild-type controls

integument phenotype

abnormal epidermis stratum corneum morphology
- however, no abnormalities are observed in the adult stratum corneum
- newborn homozygotes show an abnormally compacted stratum corneum, with fewer and smaller intercorneocyte lacunae than wild-type controls, indicating a defect in stratum corneum maturation
- the thickness of neonatal stratum corneum is reduced by 38%, whereas the thickness of the living (basal, spinous and granular/transitional) layer of the epidermis is normal

impaired skin barrier function
- Normal - however, no differences in birth weight, postnatal or long term survival are observed
- newborn homozygotes show a 54% increase in the transepidermal fluid loss rate relative to wild-type controls

short vibrissae
- at birth, homozygotes show a 30% reduction in whisker length relative to wild-type controls; however, this difference is transient, as normal size whiskers are observed in adulthood

mammalian phenotype

homeostasis/metabolism phenotype
- Normal - blood chemistry analysis revealed no significant changes in the serum levels of alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, amylase, and creatine kinase, and only a moderate increase in lactate dehydrogenase levels

integument phenotype
- Normal - no abnormalities in pelage hair eruption or pelage hair appearance are observed
- Normal - surprisingly, newborn homozygotes display normal tight junction formation and profilaggrin processing in the epidermis

References

Deltagen Inc. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data SubmissionMGI: J:101679

Additional - Tmprss13^tm1Dgen related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Tmprss13
- Genotyping resources and troubleshooting
Citation
When using the Tmprss13- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006434 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or Wild-type for Tmprss13<tm1Dgen>

Related Products and Services

Frozen Mouse Embryo	B6.129P2-Tmprss13<tm1Dgen>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6.129P2-Tmprss13<tm1Dgen>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6.129P2-Tmprss13<tm1Dgen>/J Frozen Embryos	$3373.50
Frozen Mouse Embryo	B6.129P2-Tmprss13<tm1Dgen>/J Frozen Embryos	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by academics, non-profits, companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As:Tmprss13-

Genetic overview

Research Applications

Base Price

Detailed Description

Expression Data

Selected References

Tmprss13tm1Dgen

Allele Symbol: Tmprss13tm1Dgen

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tmprss13tm1Dgen/Tmprss13tm1Dgeninvolves: 129P2/OlaHsd * C57BL/6

normal phenotype

Genotype: Tmprss13tm1Dgen/Tmprss13tm1Dgeninvolves: 129P2/OlaHsd * C57BL/6J

homeostasis/metabolism phenotype

integument phenotype

mammalian phenotype

References

Additional - Tmprss13tm1Dgen related

Genotyping Protocols

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Tmprss13^tm1Dgen

Allele Symbol: Tmprss13^tm1Dgen

Genotype: Tmprss13^tm1Dgen/Tmprss13^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6

Genotype: Tmprss13^tm1Dgen/Tmprss13^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6J

Additional - Tmprss13^tm1Dgen related