Overview

Also Known As: ChAT-IRES-Cre , ChAT-IRES-Cre::frt-neo-frt

ChAT-IRES-Cre knock-in mice express Cre recombinase in cholinergic neurons, without disrupting endogenous Chat expression. These mice may be useful in neurobiological research of motor function, learning and memory, Alzheimer's disease, and Down syndrome, as well as obesity and diabetes research.

The Jackson Laboratory Repository has two ChAT-IRES-Cre knock-in alleles from Dr. Bradford Lowell: ChAT-IRES-Cre::frt-neo-frt (Chat^tm2(cre)Lowl; Stock Nos. 006410/018957/028861) and ChAT-IRES-Cre::Δneo (Chat^tm1(cre)Lowl ; Stock No. 031661). The donating investigator reports that the presence of the neo cassette may result in ectopic Cre expression, and they have never observed any ectopic expression in their ChAT-IRES-Cre::Δneo mice (January 2018).

Donating Investigator

Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)

Genetic overview

Genetic Background	Generation
	N?+F25 (2019-03-18 00:00:00)

Chat^tm2(cre)Lowl

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Chat	choline acetyltransferase

View Genetics

Research Applications

Research Tools
Neurobiology Research

View All Research Applications

Base Price

Starting at:

$278.00 Domestic price for female 4-week

View Price List

Details

Detailed Description

The Jackson Laboratory Repository has two ChAT-IRES-Cre knock-in alleles from Dr. Bradford Lowell (Beth Israel Deaconess Medical Center / Harvard): ChAT-IRES-Cre::frt-neo-frt (Chat^tm2(cre)Lowl; Stock Nos. 006410/018957/028861) and ChAT-IRES-Cre::Δneo (Chat^tm1(cre)Lowl ; Stock No. 031661). The donating investigator reports that the presence of the neo cassette may result in ectopic Cre expression, and they have never observed any ectopic expression in their ChAT-IRES-Cre::Δneo mice (January 2018).

The strain description below summarizes the phenotype of ChAT-IRES-Cre::frt-neo-frt mice (Chat^tm2(cre)Lowl ; Stock No. 006410).
Mice that are homozygous for the ChAT-IRES-Cre::frt-neo-frt knock-in allele are viable and fertile. An "IRES-Cre" sequence is inserted downstream of the stop codon such that cre expression is controlled by the endogenous Chat gene promoter. Chat gene expression, however, is unaffected. Cre recombinase activity is reported in all cholinergic neurons. These mice may be useful for "Cre-lox" technology applications in neurobiology, including studies of motor function, learning and memory, Alzheimer's disease, and Down syndrome, and in obesity and diabetes research.

View Cre expression characterization for ChAT-IRES-Cre::frt-neo-frt allele (Chat^tm2(cre)Lowl ; Stock No. 006410).

Development

Stock No. 006410 has the ChAT-IRES-Cre::frt-neo-frt allele (Chat^tm2(cre)Lowl) on a mixed C57BL/6;129 genetic background - its generation is described below.

A targeting vector was designed to insert an optimized internal ribosome entry site-linked Cre recombinase gene (followed by an frt-flanked neomycin resistance cassette) downstream of the stop codon of the endogenous gene. The construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric mice were bred to C57BL/6 mice. The resulting mutant offspring (still retaining the frt-flanked neo) were bred to wildtype siblings, and maintained as such prior to sending heterozygous males to The Jackson Laboratory Repository in 2006. Upon arrival, mice were bred once to C57BL/6 females to rederive our living colony. Thereafter, mutant mice were bred together to make this line homozygous.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is reported in all cholinergic neurons.

Control Suggestions

Approximate Controls

101045 B6129SF2/J

Additional Information

Considerations for Choosing Controls

Selected References

Rossi J; Balthasar N; Olson D; Scott M; Berglund E; Lee CE; Choi MJ; Lauzon D; Lowell BB; Elmquist JK. 2011. Melanocortin-4 receptors expressed by cholinergic neurons regulate energy balance and glucose homeostasis. Cell Metab 13(2):195-204PubMed: 21284986 MGI: J:169562

View All References

Genetics

Chat^tm2(cre)Lowl

Allele Symbol: Chat^tm2(cre)Lowl

Allele Name	targeted mutation 2, Bradford B Lowell
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	Chat^tm2(cre)Lowl; targeted mutation 2, Bradford B Lowell
Gene Symbol and Name	Chat, choline acetyltransferase
Gene Synonym(s)	B230380D24Rik; B230380D24Rik; CHOACTASE; CMS1A; CMS1A2; RIKEN cDNA B230380D24 gene; CMS6
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is reported in all cholinergic neurons.
Strain of Origin	129S6/SvEvTac
Chromosome	14
Molecular Note	An optimized internal ribosome entry sequence (IRES) fused to cre recombinase followed by a neomycin resistance cassette flanked FRT sites was inserted after the ChAT stop codon in BAC (bMQ185k21) using homologous recombination in bacteria. A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. This allele was used to generate Chat^tm1(cre)Lowl.
Mutations Made By	Bradford Lowell, Beth Israel Deaconess Med Cntr (Harvard)

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Cancer Research
  - Cre-lox System
- Cardiovascular Research
  - Cre-lox System
- Developmental Biology Research
  - Cre-lox System
- Diabetes and Obesity Research
  - loxP
- Genetics Research
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers
  - Tissue/Cell Markers: Cre-lox System
  - Mutagenesis and Transgenesis: Cre-lox System
- Cre-lox System
  - Cre Recombinase Expression
- Neurobiology Research
Neurobiology Research
- Alzheimer's Disease
- Cre-lox System
  - Cre Recombinase expression in neural tissue
- Neurodegeneration
  - basal forebrain cholinergic neurons, Down syndrome
- Down syndrome

Mammalian Phenotype Terms by Genotype

References

Rossi J; Balthasar N; Olson D; Scott M; Berglund E; Lee CE; Choi MJ; Lauzon D; Lowell BB; Elmquist JK. 2011. Melanocortin-4 receptors expressed by cholinergic neurons regulate energy balance and glucose homeostasis. Cell Metab 13(2):195-204PubMed: 21284986 MGI: J:169562

Additional References

Bleckert A; Zhang C; Turner MH; Koren D; Berson DM; Park SJH; Demb JB; Rieke F; Wei W; Wong RO. 2018. GABA release selectively regulates synapse development at distinct inputs on direction-selective retinal ganglion cells. Proc Natl Acad Sci U S A 115(51):E12083-E12090PubMed: 30509993 MGI: J:268899
Yaeger CE; Ringach DL; Trachtenberg JT. 2019. Neuromodulatory control of localized dendritic spiking in critical period cortex. Nature 567(7746):100-104PubMed: 30787434 MGI: J:274346
Dannenberg H; Kelley C; Hoyland A; Monaghan CK; Hasselmo ME. 2019. The Firing Rate Speed Code of Entorhinal Speed Cells Differs across Behaviorally Relevant Time Scales and Does Not Depend on Medial Septum Inputs. J Neurosci 39(18):3434-3453PubMed: 30804092 MGI: J:274645
Rotterman TM; Akhter ET; Lane AR; MacPherson KP; Garcia VV; Tansey MG; Alvarez FJ. 2019. Spinal Motor Circuit Synaptic Plasticity after Peripheral Nerve Injury Depends on Microglia Activation and a CCR2 Mechanism. J Neurosci 39(18):3412-3433PubMed: 30833511 MGI: J:274670
Jin J; Cheng J; Lee KW; Amreen B; McCabe KA; Pitcher C; Liebmann T; Greengard P; Flajolet M. 2019. Cholinergic Neurons of the Medial Septum Are Crucial for Sensorimotor Gating. J Neurosci 39(26):5234-5242PubMed: 31028115 MGI: J:277413
Simon CM; Dai Y; Van Alstyne M; Koutsioumpa C; Pagiazitis JG; Chalif JI; Wang X; Rabinowitz JE; Henderson CE; Pellizzoni L; Mentis GZ. 2017. Converging Mechanisms of p53 Activation Drive Motor Neuron Degeneration in Spinal Muscular Atrophy. Cell Rep 21(13):3767-3780PubMed: 29281826 MGI: J:277586

Additional - Chat^tm2(cre)Lowl related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Chat^tm2(cre)Lowl
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony at The Jackson Laboratory Repository, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the ChAT-IRES-Cre , ChAT-IRES-Cre::frt-neo-frt mouse strain in a publication, please cite the originating article(s) and include JAX stock #006410 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available Now

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Chat<tm1(cre)Lowl>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	B6;129S6-Chat<tm2(cre)Lowl>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129S6-Chat<tm2(cre)Lowl>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6;129S6-Chat<tm2(cre)Lowl>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6;129S6-Chat<tm2(cre)Lowl>/J Frozen Embryo	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As: ChAT-IRES-Cre , ChAT-IRES-Cre::frt-neo-frt

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Chattm2(cre)Lowl

Allele Symbol: Chattm2(cre)Lowl

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional References

Additional - Chattm2(cre)Lowl related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Chat^tm2(cre)Lowl

Allele Symbol: Chat^tm2(cre)Lowl

Additional - Chat^tm2(cre)Lowl related