B6.Cg-Dab1^scm-3J/J

Stock number: 006408
Product name: scrambler(3J)
Research areas: Developmental Biology Research, Neurobiology Research

Description

These mice carry a spontaneous mutation resulting in small body size, gait instability and shortened life span.

Detailed description

By 2 weeks of age, mice homozygous for the scrambler 3 Jackson mutation are smaller than their littermates and ataxic with a severely unstable gait and inability to right themselves. They rarely survive beyond 3 weeks of age, although heterozygotes have a normal lifespan. Histology of homozygotes shows disorganization of the hippocampal layers and a lack of cerebellar folia. The scrambler 3 Jackson mutation causes a more severe phenotype than does the scrambler 2 Jackson mutation.

Development

The scrambler 3 Jackson mutation arose spontaneously in the F2 offspring of a linkage cross of B6.CAST-Cdh23^Ahl+/Kjn and B6.129S7-Sod1^tm1Leb/DnJ and was backcrossed twice to C57BL/6J before being maintained by sibling intercrossing homozygous ovarian transplant hosts with heterozygous males. The targeted mutation has been bred out of this mutant subline.

Allele

Symbol: Dab1^scm-3J
Name: scrambler 3 Jackson
MGI accession ID: MGI:3773692
Generation method: Spontaneous
Marker symbol: Dab1
Marker name: disabled 1
Chromosome: 4
Strain of origin: (B6.CAST-Cdh23⁺/Kjn x B6.129S7-Sod1^tm1Leb/DnJ)F2
Molecular note: This spontaneous mutation arose spontaneously at The Jackson Laboratory and was proven allelic with the original scrambler mutation by failed complementation.