B6;129S6-Adgrl1^tm1Sud/J

Stock number: 006393
Product name: CL1 KO
Research areas: Neurobiology Research

Description

These adhesion G protein-coupled receptor L1 ( Adgrl1, formerly Lphn1) knock-out mice are useful in studies of neurotransmission.

Detailed description

Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical abnormalities. Protein product from the targeted gene is not detected. Ca²⁺-independent binding of alpha-latrotoxin (a neurotoxic component of black widow spider venom) to brain membranes is reduced by approximately 75%. Ca²⁺-dependent binding is decreased by approximately 50%. A major reduction in alpha-latrotoxin-stimulated glutamate release is found in the presence and absence of Ca²⁺. This mutant mouse strain represents a model that may be useful in studies of neurotransmitter release mechanisms.

Development

A targeting vector containing a neomycin resistance gene was used to replace exon 2 (encoding residues 24-95) and flanking sequences of the targeted gene. The construct was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6. The strain has been minimally backcrossed to C57BL/6 by the donating laboratory.

Allele

Symbol: Adgrl1^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:3691022
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Adgrl1
Marker name: adhesion G protein-coupled receptor L1
Chromosome: 8
Strain of origin: 129S6/SvEvTac
Molecular note: Exon 2, which encodes amino acids 24-95, and some flanking intronic DNA have been replaced by a neomycin resistance cassette, generating a translational reading-frame shift. Immunoblot analysis with antibodies against the N-terminal, extracellular part of the protein demonstrated its absence in synoptosomes of homozygous mutant mice.