These adhesion G protein-coupled receptor L1 ( Adgrl1, formerly Lphn1) knock-out mice are useful in studies of neurotransmission.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical abnormalities. Protein product from the targeted gene is not detected. Ca2+-independent binding of alpha-latrotoxin (a neurotoxic component of black widow spider venom) to brain membranes is reduced by approximately 75%. Ca2+-dependent binding is decreased by approximately 50%. A major reduction in alpha-latrotoxin-stimulated glutamate release is found in the presence and absence of Ca2+. This mutant mouse strain represents a model that may be useful in studies of neurotransmitter release mechanisms.
A targeting vector containing a neomycin resistance gene was used to replace exon 2 (encoding residues 24-95) and flanking sequences of the targeted gene. The construct was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6. The strain has been minimally backcrossed to C57BL/6 by the donating laboratory.
|Allele Name||targeted mutation 1, Thomas C Sudhof|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Adgrl1, adhesion G protein-coupled receptor L1|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||Exon 2, which encodes amino acids 24-95, and some flanking intronic DNA have been replaced by a neomycin resistance cassette, generating a translational reading-frame shift. Immunoblot analysis with antibodies against the N-terminal, extracellular part of the protein demonstrated its absence in synoptosomes of homozygous mutant mice.|
|Mutations Made By|| |
Dr. Thomas Sudhof, Stanford University School of Medicine
When maintained as a live colony, either heterozygous females are bred with homozygous males, or heterozygotes are intercrossed. Homozygotes of both genders are both viable and fertile, but the females are very poor mothers.
When using the CL1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006393 in your Materials and Methods section.
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