Homozygotes are viable, fertile and have normal body weight. This strain was created by excision of a neomycin cassette from the targeted mutation line (see Stock No. 006388) and wild-type levels of expression are restored for all splice variants. These mice have no obvious neuronal phenotype.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Hypomorph, Inserted expressed sequence) | Syt7 | synaptotagmin VII |
Homozygotes are viable, fertile and have normal body weight. This strain was created by excision of a neomycin cassette from the targeted mutation line (see Stock No. 006388) and wild-type levels of expression are restored for all splice variants. These mice have no obvious neuronal phenotype.
A targeting vector containing point mutations D303A, D357A, and D359A in exon 13 as well as a floxed neomycin gene in intron 12 was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 and the neomycin gene was removed by crossing with a Cre deleter strain. The foundation line has been backcrossed at least five times to C57BL/6 by the donating laboratory.
NOTE: A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed that this strain is on a mixed genetic background (4 out of 27 markers are segregating for non-C57BL/6 alleles). July, 2008.
Allele Name | targeted mutation 2, Thomas C Sudhof |
---|---|
Allele Type | Targeted (Hypomorph, Inserted expressed sequence) |
Allele Synonym(s) | C2B KI/KO |
Gene Symbol and Name | Syt7, synaptotagmin VII |
Gene Synonym(s) | |
Promoter | Syt7, synaptotagmin VII, mouse, laboratory |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 19 |
Molecular Note | A targeting vector containing point mutations D303A, D357A, and D359A in exon 13 as well as a floxed neomycin gene in intron 12 was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. The neomycin gene was removed by crossing with a Cre deleter strain, which left the expression level at 20-30% of wild-type. |
Mutations Made By | Dr. Thomas Sudhof, Stanford University School of Medicine |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the Synaptotagmin 7 C2A/B-domain KI without Neo mouse strain in a publication, please cite the originating article(s) and include JAX stock #006389 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Syt7<tm2Sud> |
Frozen Mouse Embryo | B6;129-Syt7<tm2Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Syt7<tm2Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Syt7<tm2Sud>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Syt7<tm2Sud>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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