B6;129-Syt7^tm2Sud/J

Stock number: 006389
Product name: Synaptotagmin 7 C2A/B-domain KI without Neo
Research areas: Neurobiology Research

Description

Homozygotes are viable, fertile and have normal body weight. This strain was created by excision of a neomycin cassette from the targeted mutation line (see Stock No. 006388) and wild-type levels of expression are restored for all splice variants. These mice have no obvious neuronal phenotype.

Detailed description

Homozygotes are viable, fertile and have normal body weight. This strain was created by excision of a neomycin cassette from the targeted mutation line (see Stock No. 006388) and wild-type levels of expression are restored for all splice variants. These mice have no obvious neuronal phenotype.

Development

A targeting vector containing point mutations D303A, D357A, and D359A in exon 13 as well as a floxed neomycin gene in intron 12 was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 and the neomycin gene was removed by crossing with a Cre deleter strain. The foundation line has been backcrossed at least five times to C57BL/6 by the donating laboratory.

NOTE: A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed that this strain is on a mixed genetic background (4 out of 27 markers are segregating for non-C57BL/6 alleles). July, 2008.

Allele

Symbol: Syt7^tm2Sud
Name: targeted mutation 2, Thomas C Sudhof
MGI accession ID: MGI:3699592
Generation method: Targeted
Attributes: Hypomorph; Inserted expressed sequence
Marker symbol: Syt7
Marker name: synaptotagmin VII
Chromosome: 19
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector containing point mutations D303A, D357A, and D359A in exon 13 as well as a floxed neomycin gene in intron 12 was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. The neomycin gene was removed by crossing with a Cre deleter strain, which left the expression level at 20-30% of wild-type.