These Syt7 targeted mutant mice were developed to study the role of synaptotagmin VII in Ca2+ binding in synaptic vesicle exocytosis.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted | Syt7 | synaptotagmin VII |
There is no obvious neuronal phenotype. Western analysis indicates that no protein product is produced by the mutant allele which carries point mutations D225A, D227A, and D233A in exon 11 (previously identified as exon 12), and mutations D303A, D357A, and D359A in exon 13, along with a floxed neomycin resistance gene. There is no obvious neuronal phenotype. We have been unable to recover viable homozygous pups in our colony, but viability/fertility has been noted by the donating laboratory.
A targeting vector containing point mutations D225A, D227A, and D233A in exon 11 (previously identified as exon 12), and mutations D303A, D357A, and D359A in exon 13, along with a floxed neomycin resistance gene in the intervening intron was produced. The vector was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 and the foundation line has been backcrossed at least five times to C57BL/6 by the donating laboratory.
NOTE: A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed that this strain is on a mixed genetic background (9 out of 27 markers are segregating for non-C57BL/6 alleles). July, 2008.
Allele Name | targeted mutation 1, Thomas C Sudhof |
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Allele Type | Targeted |
Allele Synonym(s) | C2AB KI/KO |
Gene Symbol and Name | Syt7, synaptotagmin VII |
Gene Synonym(s) | |
Promoter | Syt7, synaptotagmin VII, mouse, laboratory |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 19 |
Molecular Note | Point mutations D225A, D227A, and D233A in exon 11 (previously identified as 12), and mutations D303A, D357A, and D359A in exon 13, along with a floxed neomycin resistance gene in the intervening intron were introduced in the Syt7 locus. No protein is detected by Western blot. |
Mutations Made By | Dr. Thomas Sudhof, Stanford University School of Medicine |
When maintained as a live colony, heterozygotes may be bred. We have been unable to recover viable homozygous pups in our colony.
When using the Synaptotagmin-7 mouse strain in a publication, please cite the originating article(s) and include JAX stock #006388 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Syt7<tm1Sud> |
Frozen Mouse Embryo | B6;129-Syt7<tm1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Syt7<tm1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Syt7<tm1Sud>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Syt7<tm1Sud>/J Frozen Embryo | $3373.50 |
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