B6;129-Syt7^tm1Sud/J

Stock number: 006388
Product name: Synaptotagmin-7
Research areas: Neurobiology Research

Description

These Syt7 targeted mutant mice were developed to study the role of synaptotagmin VII in Ca²⁺ binding in synaptic vesicle exocytosis.

Detailed description

There is no obvious neuronal phenotype. Western analysis indicates that no protein product is produced by the mutant allele which carries point mutations D225A, D227A, and D233A in exon 11 (previously identified as exon 12), and mutations D303A, D357A, and D359A in exon 13, along with a floxed neomycin resistance gene. There is no obvious neuronal phenotype. We have been unable to recover viable homozygous pups in our colony, but viability/fertility has been noted by the donating laboratory.

Development

A targeting vector containing point mutations D225A, D227A, and D233A in exon 11 (previously identified as exon 12), and mutations D303A, D357A, and D359A in exon 13, along with a floxed neomycin resistance gene in the intervening intron was produced. The vector was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 and the foundation line has been backcrossed at least five times to C57BL/6 by the donating laboratory.

NOTE: A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed that this strain is on a mixed genetic background (9 out of 27 markers are segregating for non-C57BL/6 alleles). July, 2008.

Allele

Symbol: Syt7^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:3699518
Generation method: Targeted
Marker symbol: Syt7
Marker name: synaptotagmin VII
Chromosome: 19
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Point mutations D225A, D227A, and D233A in exon 11 (previously identified as 12), and mutations D303A, D357A, and D359A in exon 13, along with a floxed neomycin resistance gene in the intervening intron were introduced in the Syt7 locus. No protein is detected by Western blot.