B6;129P2-Sv2a^tm1Sud Sv2b^tm1Sud/J

Stock number: 006383
Product name: SV2A/SV2B double KO
Research areas: Neurobiology Research

Description

Surviving SV2A/SV2B double KO mice are subject to seizures and are useful in studies of neuronal synaptic vesicles.

Detailed description

Approximately half of the double homozygous targeted mutation mice die within days of birth. The surviving mice are subject to severe seizures that start in the second postnatal week then die within the following 2 weeks. Nissl staining of brain tissues revealed no structural abnormalities. No changes were found in the levels of selected synaptic proteins. Neurons experience sustained increases in Ca²⁺-dependent synaptic transmission when two or more action potentials are triggered in succession. Electrophysiological recordings from cultured hippocampal cells exhibit no detectable abnormalities. No protein products from the targeted genes are detected in brain tissue. This mutant mouse strain represents a model that may be useful in studies of synaptic vesicle trafficking in neurons.

Development

Targeting vectors containing a neomycin resistance gene were used to replace exons 2-9 encoding residues 208-559 of Sv2a and exon 1 encoding residues 1-149 of Sv2b. The constructs were electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6. The strain has been minimally backcrossed to C57BL/6 by the donating laboratory.

Allele

Symbol: Sv2b^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:2179737
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Sv2b
Marker name: synaptic vesicle glycoprotein 2 b
Chromosome: 7
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin selection cassette replaced the first coding exon and flanking sequences. Western blot analysis on brain lysates derived from homozygous mice confirmed that no detectable encoded protein was made.

Allele

Symbol: Sv2a^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:2179736
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Sv2a
Marker name: synaptic vesicle glycoprotein 2 a
Chromosome: 3
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin resistance cassette replaced a genomic fragment containing exons 2-9. Western blot analysis on brain lysates derived from homozygous mice demonstrated that no detectable protein was expressed from this allele.