Surviving SV2A/SV2B double KO mice are subject to seizures and are useful in studies of neuronal synaptic vesicles.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Sv2a | synaptic vesicle glycoprotein 2 a |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Sv2b | synaptic vesicle glycoprotein 2 b |
Approximately half of the double homozygous targeted mutation mice die within days of birth. The surviving mice are subject to severe seizures that start in the second postnatal week then die within the following 2 weeks. Nissl staining of brain tissues revealed no structural abnormalities. No changes were found in the levels of selected synaptic proteins. Neurons experience sustained increases in Ca2+-dependent synaptic transmission when two or more action potentials are triggered in succession. Electrophysiological recordings from cultured hippocampal cells exhibit no detectable abnormalities. No protein products from the targeted genes are detected in brain tissue. This mutant mouse strain represents a model that may be useful in studies of synaptic vesicle trafficking in neurons.
Targeting vectors containing a neomycin resistance gene were used to replace exons 2-9 encoding residues 208-559 of Sv2a and exon 1 encoding residues 1-149 of Sv2b. The constructs were electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6. The strain has been minimally backcrossed to C57BL/6 by the donating laboratory.
Allele Name | targeted mutation 1, Thomas C Sudhof |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | SV2A- |
Gene Symbol and Name | Sv2a, synaptic vesicle glycoprotein 2 a |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 3 |
Molecular Note | A neomycin resistance cassette replaced a genomic fragment containing exons 2-9. Western blot analysis on brain lysates derived from homozygous mice demonstrated that no detectable protein was expressed from this allele. |
Allele Name | targeted mutation 1, Thomas C Sudhof |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | SV2B- |
Gene Symbol and Name | Sv2b, synaptic vesicle glycoprotein 2 b |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | A neomycin selection cassette replaced the first coding exon and flanking sequences. Western blot analysis on brain lysates derived from homozygous mice confirmed that no detectable encoded protein was made. |
When maintaining a live colony, mice heterozygous for both mutations may be bred together. Once established, mice heterozygous for the Sv2a mutation and homozygous for Sv2b mutation may be bred together (or to wildtype mice). Mice homozygous for both mutations are not viable.
When using the SV2A/SV2B double KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006383 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Sv2a<tm1Sud>, Heterozygous or Wild-type for Sv2b<tm1Sud> |
Frozen Mouse Embryo | B6;129P2-Sv2a<tm1Sud> Sv2b<tm1Sud>/J | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Sv2a<tm1Sud> Sv2b<tm1Sud>/J | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Sv2a<tm1Sud> Sv2b<tm1Sud>/J | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Sv2a<tm1Sud> Sv2b<tm1Sud>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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